N-乙酰半胱氨酸对急性肺损伤大鼠保护作用.doc

N-乙酰半胱氨酸对急性肺损伤大鼠保护作用 　【摘要】 　　目的： 探讨N-乙酰半胱氨酸（NAC）对油酸(OA)诱导的急性肺损伤（ALI）大鼠的保护作用。方法： 成年雄性SD大鼠18只，随机分成对照组、损伤组（ALI组）和N-乙酰半胱氨酸组（NAC组），后两组在注射OA后3 h检测肺湿重与肺干重比（W/D），肺组织匀浆髓过氧化物酶（MPO）、超氧化物歧化酶（SOD）、丙二醛（MDA）及肺组织病理检查。结果： ALI组MPO、 MDA明显升高，SOD明显降低，与对照组比较有明显差异（Plt;0.01），NAC组MPO、MDA低于ALI组，SOD明显升高，Plt;0.01；ALI组MPO 水平与肺组织病理损伤程度成正相关(r=0.793,Plt;0.01)；肺组织病理组织学显示，NAC预处理能明显改善肺组织病理损伤。结论: NAC对ALI大鼠具有保护作用，其保护机制可能与清除氧自由基有关。 【关键词】 急性肺损伤 N-乙酰半胱氨酸 氧自由基 过氧化物酶类 超氧化物歧化酶 丙二醛 　　［Abstract］ Objective: To investigate the protective effect of Nacetylcysteine (NAC) on lung injury in rats induced by oleic acid (OA). Methods: Eighteen male SpragueDawley rats were randomly divided into acute lung injury (ALI) group (n=6), Nacetylcysteine (NAC) group (n=6) and control group (n=6), The lung tissues were collected in 3h after OA or saline administration. Lung weight/dry ratio(W/D), the concentrations of lung homogenate myeloperoxidase (MPO), malondiadehyde (MDA), and superoxide dismutase (SOD) were measured. Meanwhile, The pathological changes of lung were examined with light microscope. Results: In ALI group, levels of lung homogenates MPO, MDA were significantly higher and the level of SOD were significantly lower than those in control group and NAC group(All Plt;0.01). The levels of MPO in rats of ALI group have a significantly positive correlation with lung pathological injury scores (r=0.793, plt;0.01). Histopathologic findings showed less lung injury in NAC group than in ALI group. Conclusions: NAC might have effect of alleviating the lung injury induced by oleic acid , of which the mechanism might be associated with free oxygen radical elimination. 　　［Key words］ acute lung injury; Nacetylcysteine; peroxidases; superoxide dismutase; malondialdehyde 　　急性肺损伤（Acute Lung Injury,ALI）是由脓毒血症、创伤和休克等多种病因引起的全身炎症反应综合症（systemic inflammatory response syndrome, SIRS）在肺部的表现，病死率34％～60％。目前国内外对ALI的药物干预研究较多，但临床研究结果均未能取得满意的疗效。N-乙酰半胱氨酸（Nacetylcysteine，NAC）是一种经典的祛痰药，不仅具有溶解黏液作用，而且具有较强抗氧化、抗炎作用[1]。本文以ALI大鼠模型为研究对象，观察NAC对ALI的