丙烯酰胺对雄性大鼠生殖损伤及机制探究.docVIP

丙烯酰胺对雄性大鼠生殖损伤及机制探究 　 作者：段志文，张玉敏，裴秀丛 【摘要】 研究丙烯酰胺对雄性大鼠生精功能及睾丸的损害作用，探讨产生雄性生殖毒性的原因及CYP2E1的作用。方法：选择健康成熟的雄性Wistar大鼠60只，随机分为阴性对照组（生理盐水）、ACR 20mg/kg组、诱导剂无水乙醇200mg/kg组、无水乙醇200mg/kg+ ACR 20mg/kg组、抑制剂二乙基二硫代氨荃甲酸钠(DDC)20mg/kg组、DDC20mg/kg+ ACR 20mg/kg共6组，以5ml/kg容量连续灌胃染毒4周。检测睾丸、附睾、前列腺、精囊腺脏器系数和睾丸病理；血中睾酮（T）、雌二醇（E2）含量；睾丸组织GSH、GR、GSHPx水平；精子数，精子畸形率；曲细精管第Ⅶ、Ⅷ期各细胞成分构成变化。结果：ACR、ACR+诱导剂及ACR+抑制剂组精子数下降、畸形率升高，与阴性对照组比较存在显著差异，GSH含量显著升高（Plt;0.05），其它指标均无显著变化（Pgt;0.05）。结论：ACR可引起大鼠生精功能的改变，但未观察到CYP2E1在这种改变中的明显作用。 【关键词】 丙烯酰胺；雄性生殖毒性；机制 　　AbstractObjective：To study the effects and the mechanisms of acrylamide(ACR) on the spermatogenesis and testis in male rats and explore the effect of CYP2E1.Methods：Sixty Wistar male rats were divided equally into six groups stochasticly, including control group (NS) and five test substances treatment groups (ACR 20mg/kg, inductor alcohol 200mg/kg, ACR 20mg/kg+alcohol 200mg/kg, inhibitor sodium diethyl dithiocarbamate(DDC) 20mg/kg, ACR 20mg/kg+DDC 20mg/kg respectively).Rats were given gastric gavage in 5ml/kg one time everyday for four weeks. The organ coefficients of testis, epididymis, prostate, seminal vesicle were measured as well as the levels of GSH, GR, GSH-Px in testis homogenate. The levels of testosterone and estradiol in serum were detected by RIA. Spermatozoa and teratospermia were counted. It was analysed that the cellular components of primary spermatocyte, prespermatid and spermatoon in seminiferous tubules Ⅶ/Ⅷ stage.Results：Compared with control group, spermatozoa count decreased significantly and teratospermia and GSH levels increased significantly in ACR, ACR+alcohol and ACR+DDC groups, Other indexes did not change significantly.Conclusion：ACR changes spermatogenesis in male rats, but not via the pathway of CYP2E1. 　　Key wordsAcrylamide,male reproductive toxicity;mechanism 丙烯酰胺（acrylamide, ACR）作为一种工业原料，应用广泛。日常生活中以淀粉为主要成分的食品经高温加工处理后可含有大量ACR，因此，ACR的毒性研究引起广泛关注。动物实验已经证实，ACR具有神经毒性、发育毒性及动物致癌性[1,2],其对生殖损害作用也已确认[3]，但机制不明。有人认为，ACR在体内经CYP2E1代谢生成的环氧ACR，其毒性高于