外周血NK，IL12，CRP在子痫前期中测定及意义.docVIP

外周血NK，IL12，CRP在子痫前期中测定及意义 　【摘要】 目的 测定子痫前期孕妇外周血NK、IL12、CRP水平的变化并探讨其意义。方法 选择子痫前期患者42例，正常晚孕组20例，正常未孕育龄妇女20例，测定外周血NK细胞、IL12及CRP水平并行统计学分析。结果 子痫前期组NK细胞平均值及IL12，CRP水平明显高于正常晚孕组和正常未孕组；各组血中NK细胞值与IL12水平呈正相关关系，NK与CRP之间相关性不明显。结论 NK细胞参与了子痫前期的发病，IL12通过激活NK细胞，诱发免疫介导性血管内皮炎症、损伤引起子痫前期疾病；CRP水平在子痫前期时显著增加，可能成为临床早期发现子痫前期发病的一个灵敏指标。 【关键词】 子痫前期；杀伤细胞，天然；白细胞介素12；C反应蛋白 　　 　　To explore the changes of NK, IL12 and CRP levels in pregnancy with preeclampsia and its clinical significance. Methods The NK cells, IL12 and CRP levels were determined in the peripheral blood of 42 pregnant women with preeclampsia, 20 normal late pregnant women and 20 normal nonpregnant women. Results Percentages of NK cells and levels of IL12 and CRP were higher in the preeclampsia group than in the late pregnant group and the nonpregnant group. There was a positive correlation between percentage of NK cells and level of IL12 and no evident correlation between NK cells and CRP. Conclusions NK cells participate in the onset of preeclampsia. IL12 intensifies immunological rejection on the maternalfetal interface by activating the NK cells and induces immunemediated damage of blood vessel endothelium and inflammatory reaction, which results in preeclampsia. CRP, which is increased in pregnancy with preeclampsia, may be a sensitive biochemical marker for early diagnosing of preeclampsia. 　　Key words: PreEclampsia; Killer cell, natural; Interleukin12; Creactive protein 　　子痫前期（preeclampsia，PE）是严重威胁母儿健康的妊娠期特有疾病，虽然发病机制目前尚未完全清楚,但血管内皮损伤是其中心环节。近年来，由于分子免疫学的发展，免疫介导的炎性损伤在PE发病机制中所起的作用越来越受到人们的重视。本研究对晚孕期PE孕妇外周血自然杀伤（Natural Killer,NK）细胞水平及白介素12 (Interleukin12, IL12)及C反应蛋白（CReactive Protein, CRP）水平的变化进行检测,初步探讨了NK细胞、IL12及CRP在子痫前期发病中的可能作用及相互关系，为能从免疫学角度寻找防治此病的措施提供新的思路。 　　1 资料与方法 　　1.1 标本来源及分组 　　选择2006年9月至2007年2月在山东省立医院产科住院的子痫前期患者42例（Preeclampsia Pregnancy,PE组）。选择同期住院的正常晚孕妇女（Normal Late Pregnancy，NLP组）20例为实验对照组，两组产次匹配，均为单胎妊娠。选择同期正常已婚育龄妇女20例作为正常对照组（Normal Nonpregnancy, NNP组）。各组病例既往无特殊疾病史，未服用免疫抑制或增强药物。 　　1.2 标本