大鼠肝脏撞击伤动物模型制备及评价.docVIP

大鼠肝脏撞击伤动物模型制备及评价 　作者：陆明，汤礼军，田伏洲，麻晓林 【摘要】 目的 建立一种新型大鼠肝脏钝性撞击伤模型，并进行评价。方法 健康SD大鼠42只，随机分为3组(n=14)。通过增加了T型二次打击头的改良型生物撞击机，用轻、中、重3种质量的钢球分别撞击1组大鼠，复制出3组损伤程度不同的大鼠肝脏钝性撞击伤模型，即轻度致伤组、中度致伤组、重度致伤组。各组再按观察指标不同，随机平分为A组和B组。测定大鼠A组致伤前后生命体征变化及自然存活数，B组致伤前后血清中谷草转氨酶(AST)、谷丙转氨酶(ALT)含量及致伤后腹腔内出血量。所有大鼠死亡后均进行损伤分级并进行评分。结果 各组平均股动脉压致伤后均低于致伤前(Plt;0.01)，且致伤后平均股动脉压随损伤程度的增加而降低(Plt;0.01或Plt;0.05);各组AST、ALT含量致伤后均明显高于致伤前(Plt;0.01或Plt;0.05)，致伤后各组AST、ALT含量均随损伤程度的增加而增高(Plt;0.01或Plt;0.05);各组腹腔出血量随损伤程度的增加而增多(Plt;0.01或Plt;0.05);各组肝脏损伤评分随损伤程度的增加而增高(Plt;0.01)。结论 各组大鼠肝脏损伤程度随钢球质量的增加而加重，分级明显。该大鼠肝脏创伤模型建模过程简单，具有稳定的肝脏损伤的伤情特点,可重复性好,损伤程度均一并可分级调节，费效比低，是一种比较理想的动物实验模型。 【关键词】 肝脏损伤;撞击伤;动物模型 　　Abstract: Objective To establish and evaluate a new type model of blunt impact injury of liver in rats.Methods Totally 42 healthy SpragueDawley rats were randomly divided into 3 groups(mild injury group,moderate injury group and severe injury group).By the improved biological impact machine with a Ttype secondhit head,3 groups of hepatic bluntimpact injury model were established respectively with three differentweight steel balls.Then each group was randomly divided into A group and B group on account of the difference of items measured.The difference in vital signs before and after injury and the number of natural survival animals after injury in A group were measured.AST,ALT content in serum before and after injury and the amount of intraabdominal hemorrhage of animals after injury in B group were measured.The injury of all rats was classified and given a score after the death.Results The average femoral artery pressure after injury in each group was significantly lower than that before injury(Plt;0.01),and decreased as the degree of injury increased(Plt;0.01 or Plt;0.05).The serum levels of AST,ALT concentration after injury in each group were significantly higher than those before injury(Plt;0.01 or Plt;0.05),and increased with degree of injury(Plt;0.01 or Plt;0.05).The amount of intraabdominal hemorrhage in each group signifi