实验性脑出血大鼠脑组织形态学动态变化.docVIP

实验性脑出血大鼠脑组织形态学动态变化 　 作者：王丹，李平友，郑勇，柯岩，段丽欢，王秋桂，殷妮娜，陈业文 【摘要】 目的 研究大鼠脑出血后神经元改变及胶质增生的动态变化特征以及变化规律对行为学的影响。方法 将SD大鼠随机分为正常对照组、假手术组、出血组（分12h组、24h组、48h组、72h组4个时相组，每组5只）。采用自体股动脉血注入尾状核建立大鼠脑出血模型，通过对脑血肿周围脑组织坏死神经元计数和Longa行为学评分，动态观察各组大鼠脑血肿周围组织形态学的变化及神经功能状态。结果 脑出血组12h即出现血肿周围脑组织部分神经元变性坏死，少数炎性细胞浸润，48h达高峰，72h后可见血肿周围胶质细胞、血管增生。脑出血组各时点的神经功能障碍评分均高于正常对照组和假手术组（P均lt;0.05）。结论 脑出血后神经元变性、坏死，胶质细胞和血管增生可能是脑水肿加重和病情加重的主要原因之一；神经行为学变化与神经元病变及神经胶质细胞的增生性反应之间存在明显相关。 【关键词】 脑出血；形态学；行为；大鼠 ABSTRACT:Objective To dynamically observe the neuronal changes and gliosis hyperplasia in perihematoma，and the behavioral changes after Intracerebral Hemorrhage(ICH) in rats.Methods The SD rats were divided into control group, sham operation group and ICH group.All the rats of the ICH group were assessed at 12h,24h,48h,72h after ICH.The intracerebral hemorhage model was established by injecting autononanticoagulated artery blood into the caudate nucleus of the rats.Neurobehavioral outcome was assessed in perihematoma.Results Some neurons displayed necrosis and few inflammatory cells were observed in the perihematoma region at 12 hours after ICH,reached to the top at 48 hours.At 72 hours after ICH gliosis and capillary hyperplasia were observed around hematoma. Neurobehavioral scores at various stages in ICH group were higher than those in control and sham group（Plt;0.05）.Compared with control group,there were nerve cell necrosis and glia cell hyperplasia. Conclusion Neurobehavioral change closely was related to both nerve cell necrosis and hyperplasia reaction of glia cell. KEY WORDS：Intracerebral hemorrhage;Histomorphology;Behavior;Rat 脑出血(intracerebral hemorrhage,ICH)是自发的非外伤性脑实质内出血。其发病率高、死亡率高、致残率高[1]。ICH后脑水肿形成是导致脑损伤的重要环节。急性期血肿扩大和脑水肿是导致ICH病情加重的主要原因，控制继续出血和减轻脑水肿是治疗ICH的关键。但水肿的发生和发展机制目前仍不清楚。探讨ICH规律及其机制，对临床上治疗和控制病情的加重至关重要。为此，我们应用脑立体定向技术，用大鼠自体动脉血建立脑出血模型，动态观察其行为学及血肿周围脑组织形态学变化。 1 材料和方法 1.1 实验仪器 江湾Ⅱ型立体定向仪；微量注射器；显微外科手术器械；牙科钻；德国leica（RM2245）石蜡切片机；Motic（BA300）数码摄像系统和olympus（CH2）生物显微镜。 1.2 实验动物及分组 健康成年SD雄性大鼠60只，体质量220～250g，由