小檗碱抑制兔胆固醇结石形成作用及机制.docVIP

小檗碱抑制兔胆固醇结石形成作用及机制 　 作者：王江华　陈先祥　蔡庆和　程彩涛　何志军　吴liming 【摘要】 　　目的 观察小檗碱对兔胆囊胆固醇结石形成的预防作用和机制。方法 动物模型：日本大耳白兔30只，随机等分三组：每组10只，对照组喂普通饮食；结石组，喂含1.2%高胆固醇饲料100g；实验组，同时喂饲含1.2%高胆固醇饲料100 g和每日10 mg/kg的小檗碱灌胃。8周后利用全自动生化分析仪酶法测定兔胆汁中的总胆固醇、磷脂、胆盐，通过Carey表计算胆固醇饱和指数(cholesterol saturation index，CSI)，观察各组胆囊胆汁结晶情况及环氧合酶-2 (cyclooxygenase2，COX2)含量。结果 实验组比成石组形成胆固醇结晶机会明显减少(8／10、3／10， ?字2=5.051，Plt;0.05)；各组胆囊胆汁中CSI (对照组0. 82±0.23、结石组1.64 ±0.27 及实验组1.13±0.29)；结石组COX2表达与空白组相比明显升高(47.66±1.54比28.39±1.46，Plt;0．05)，实验组较之结石组COX2表达量有降低(37.42±2.45比47.66±1.54，Plt;0.05)。结论 小檗碱能够抑制胆固醇结石形成和发展，其机制与调节脂质代谢，改善致石性胆汁及抑制COX2的活性等作用有关。 【关键词】 胆固醇结石； 盐酸小檗碱； 环氧合酶-2；兔 　　 Inhibitory effects of berberine on the formation of cholesterol gallstone and its mechanism in rabbits Abstract Objective To investigate and observe the inhibitory effects of berberine on the formation of experimental gallstone and explore its possible mechanism． Methods Three dietary groups of Japanese rabbits with 10 rabbits in each group were allocated as control （fed with normal diet）， lithogenic (fed with 1.2 g high cholesterol) and berberine prevention (fed with 1.2 g high cholesterol diet +10 berberine mg/kg/day) groups respectively for 8 weeks. Check the level of TC， PL， BS， bile was obtained for observing gallstone formation， and the morphologic changes of the mucosa were assessed under the microscope. The level of COX2 in gallbladder mucosa in each group was estimated with immunohistochemical assay. Results The opportunity of the formation of the cholesterol crystall in the berberine group was obviously less than those in gallstone group to form gallstone(3/ 10 vs 8/10， Plt;0.05). CSI in each group was as follow：(control 0.82±0.23， lithogenic 1.64±0.27 and berberine prevention 1.13±0.29)， respectively. The degree of mucosal inflammation and level of COX2 expression were significantly higher in gallstone group than that in the other two groups (47.66±1.54 vs 28.39±1.46， Plt;0.05； 37.42±2.45 vs 47.66±1.54， Plt;0.05). Conclusions Berberine can inhi