小檫碱对高脂兔模型脂代谢及脂肪PPARγ、INSIG2基因表达影响.docVIP

小檫碱对高脂兔模型脂代谢及脂肪PPARγ、INSIG2基因表达影响 　 作者：何琦 金磊 周岐新 杨俊霞 【摘要】 　　目的 研究小檗碱对高脂兔模型脂代谢的影响，及其与脂肪代谢相关基因表达变化的相关性。方法 采用高脂饲料喂养建立家兔高脂模型，测定各处理组血清TC、TG、LDLC和HDLC水平。实时荧光定量PCR技术检测脂肪组织PPARγ和INSIG2基因表达。 结果 高脂组TC、TG和LDLC水平较普食组明显升高（Plt;0.05），而小檗碱处理后血清TC、TG和LDLC的水平较高脂组明显降低（Plt;0.05）；实时荧光定量PCR检测结果显示，高脂组PPARγ和INSIG2 mRNA表达明显高于普食组（Plt;0.05），小檗碱处理后PPARγ mRNA表达明显较高脂组显著降低（Plt;0.05），而INSIG2 mRNA表达则较高脂组明显上调（Plt;0.05），且低剂量作用更明显。 结论 小檗碱具有明显的降血脂作用，其机制与PPARγ表达下调而INSIG2基因表达上调有关。 【关键词】 小檗碱；高血脂；PPARγ；INSIG2 【Abstract】Objective To investigate the effects of berberine on lipids metabolism of hyperlipidemic rabbits, and to explore whether its mechanism was involved in the regulation of PPARγ and INSIG2 gene expressions in adipose tissue. Methods The rabbit hyperlipidemic model was established by feeding with high fat diet. The levels of serum total cholesterol (TC), triglycerides (TG), low density lipoproteincholesterol (LDLC) and high density lipoproteincholesterol (HDLC) in each different treated group were determined. The mRNA expressions of PPARγand INSIG2 in adipose tissue were quantified by real time quantitative PCR. Results The levels of serum TC, TG and LDLC in high fat diet group obviously were elevated compared with those in normal diet group (P＜0.05). Compared with those of the high fat diet group, the serum levels of TC, TG and LDLC in berberine treated group were significantly decreased (P＜0.05). The result of real time PCR demonstrated that the mRNA expressions of PPARγand INSIG2 in high fat diet group were significantly higher than those of the normal diet group (P＜0.05), while the PPARγmRNA expression was inhibited and the INSIG2 mRNA expression was upregulated by berberine (P＜0.05). Conclusions Berberine can obviously decrease the blood lipid levels in the hyperlipidemic rabbits and its mechanisms may be related to the downregulation of the PPAγmRNA expression and upregulation of the INSIG2 mRNA expressions. 【Key words】Berberine; Hyperlipidemia; PPARγ; INSIG2