建立卵巢过度刺激综合征大鼠模型探究.docVIP

建立卵巢过度刺激综合征大鼠模型探究 　【摘要】 目的 构建卵巢过度刺激综合征(OHSS)的大鼠模型。方法 32只未成年Wistar雌鼠，随机分为常规刺激组和过度刺激组。过度刺激组：22日龄Wistar雌鼠连续4天皮下注射含有10 IU PMSG的生理盐水0.2ml，至26日龄皮下注射含有30 IU hCG的生理盐水0.2ml。设立对照组为常规刺激组：24日龄Wistar雌鼠皮下注射含有10IU PMSG的生理盐水0.2ml，再予皮下注射含有10IU hCG的生理盐水0.2ml。测血E2水平，尾静脉注入美兰溶液30min后取腹腔灌洗液和卵巢，用分光光度计分别测定腹腔灌洗液和卵巢组织中的美兰含量来判断腹膜和卵巢血管通透性；比较两组间卵巢重量，血E2水平及卵巢、腹腔液中美兰含量。结果 注射hCG 48h后，过度刺激组大鼠卵巢重量、血E2水平、腹腔液和卵巢中美兰含量均显著高于常规刺激组。结论 利用适当日龄Wister大鼠可以成功构建OHSS动物模型。 【关键词】 卵巢过度刺激综合征；动物模型；大鼠 ABSTRACT：Objective To establish the rat model of ovarian hyperstimulation syndrome(OHSS).Methods Immature female Wistar rats aged 22 days were divided into two groups: the OHSS group (16 rats) was given 10 IU PMSG for 4 consecutive days and given 30 IU hCG on 26dayold to produce ovarian hyperstimulated manifestations; the conventional stimulation group (16 rats) was given 10 IU PMSG on 24dayold and given 10 IU hCG on 26dayold. On 28dayold, the rats were injected via the tail vein Methylene blue dye solution to evaluate the capillary permeability in the peritoneal cavity and ovary; blood samples were collected from the inferior caval vein to evaluate serum estradiol(E2). Results Compared with the conventional group,the contents of methylene blue in peritoneal cavity and ovarian were significantly higher in the OHSS group; Ovarian wet weights and serum concentration of E2 were also markedly increased. Conclusion The animal model of OHSS can be induced by injected PMSG and hCG on immature female Wistar rats. KEY WORDS：Ovarian hyperstimulation syndrome(OHSS); Animal model; Rat 卵巢过度刺激综合征(ovarian hyperstimulation syndrome ,OHSS)是辅助生育技术中超促排卵时发生的一种医源性并发症，OHSS主要病理表现为卵巢增大，多发性滤泡囊肿及黄体囊肿形成；病理生理机制主要为全身毛细血管通透性增加；临床表现为胃肠道不适、腹水、胸水、少尿、卵巢增大等，严重可危及生命。为了方便更深入地对OHSS发病的具体机制及药物治疗效果进行研究，人们建立了多种OHSS的动物模型。本文主要研究国产孕马血清促性腺激素（pregrant mate’s serum gonadotropin,PMSG)和人绒毛膜促性腺激素(human chorionic gonadotropin,HCG)在未成年Wistar大鼠建立OHSS模型的可行性。 1 材料与方法 1．1 动物及分组 清洁级雌性Wistar大鼠32只，22日龄，体重49～58g,由华中科技大学同济医学院实验动物中心提供。黑暗12h，光照12h。大鼠随机分为两组：过度刺激组，常规刺激组，每组