il-12 rb1 genetic variants contribute to human susceptibility to severe acute respiratory syndrome infection among chinese白介素rb1基因变异导致人类对严重急性呼吸系统综合症感染在中国.pdfVIP

IL-12 RB1 Genetic Variants Contribute to Human Susceptibility to Severe Acute Respiratory Syndrome Infection among Chinese 1. 2. 1 3 4 2 1 Fang Tang , Wei Liu , Fang Zhang , Zhong-Tao Xin , Mao-Ti Wei , Pan-He Zhang , Hong Yang , Hinh Ly3, Wu-Chun Cao1,2* 1 Beijing Institute of Microbiology and Epidemiology, Beijing, People’s Republic of China, 2 State Key Laboratory of Pathogen and Biosecurity, Beijing, People’s Republic of China, 3 Pathology and Laboratory Medicine Department, Emory University School of Medicine, Atlanta, Georgia, United States of America, 4 Affiliated Hospital of APFCP Medical College, Tianjin, People’s Republic of China Abstract Background: Cytokines play important roles in antiviral action. We examined whether polymorphisms of interleukin (IL)-12 receptor B1 (IL-12RB1) affect the susceptibility to and outcome of severe acute respiratory syndrome (SARS). Methods: A case-control study was carried out in Chinese SARS patients and healthy controls. The genotypes of 4SNPs on IL-12 RB1 gene, +705A/G,+ 1158T/C, + 1196G/C and + 1664 C/T, were determined by PCR-RFLP. Haplotypes were estimated from the genotype data using the expectation-maximisation algorithm. Results: Comparison between patients and close contacts showed that individuals with the + 1664 C/T (CT and TT) genotype had a 2.09-fold (95% confidence interval [CI], 1.90–7.16) and 2.34-fold (95% CI, 1.79–13.37) increased risk of developing SARS, respectively. For any of the other three polymorphisms, however, no significant difference can be detected in allele or genotype frequencies between patients and controls. Additionally, estimation of the frequencies of multiple-locus haplotypes revealed potential risk hap