the ototoxic effect of intratympanic terbinafine applied in the middle ear of rats的耳毒性的效应intratympanic terbinafine应用中耳的老鼠.pdfVIP

Sagit et al. Journal of Otolaryngology - Head and Neck Surgery 2013, 42:13 /content/42/1/13 ORIGINAL RESEARCH ARTICLE Open Access The ototoxic effect of intratympanic terbinafine applied in the middle ear of rats 1* 1 1 2 Mustafa Sagit , Mehmet Akıf Somdas , Ferhat Korkmaz and Alper Akcadag Abstract Background: Otomycosis is defined as an infection of the external ear canal with fungal agents. The treatment of the disease is cleansing and drying of the external ear canal, identification and treatment of any predisposing factors and application of topical antifungal agents. Terbinafine is used as an antifungal agent to treat otomycosis. We proposed to investigate the probable ototoxic effect of terbinafine solution on auditory brain stem response (ABR) and distortion product otoacoustic emission (DPOAE) when applied intratympanically in the middle ear of rats. Methods: The experiment was performed on 30 female Wistar albino rats. Thirty animals were divided into three groups of 10 animals each. 1% terbinafine solution was administered to the first group (group T). The second group (group G) was administered 40 mg/ml gentamicin solution (ototoxic control). The third group (group S) was administered saline solution (negative control). Baseline DPOAE measurements and ABR testing from the left ears were obtained from the animals in all groups under general anesthesia. Ear solutions were applied in the middle ear intratympanically with a dental needle. Treatment was initiated after baseline measurements and repeated once every two days for fifteen days. Results: Pre and post-treatment DPOAE responses for all tested frequencies of group T and Group S showed no statistically significant difference. However, the group