the structural complexity of the human boris gene in gametogenesis and cancer人类鲍里斯在配子形成基因的结构复杂性和癌症.pdfVIP

The Structural Complexity of the Human BORIS Gene in Gametogenesis and Cancer 1 1 2 1 1 Elena M. Pugacheva *, Teruhiko Suzuki , Svetlana D. Pack , Natsuki Kosaka-Suzuki , Jeongheon Yoon , 3 4 1 1 Alexander A. Vostrov , Eugene Barsov , Alexander V. Strunnikov , Herbert C. Morse III , Dmitri Loukinov1, Victor Lobanenkov1 1 Laboratory of Immunopathology, National Institute of Allergy and Infectious Disease, National Institutes of Health (NIH), Rockville, Maryland, United States of America, 2 Chromosome Pathology Unit, Laboratory of Pathology, Center for Cancer Research (CCR), National Cancer Institute (NCI), National Institutes of Health (NIH), Bethesda, Maryland, United States of America, 3 Department of Psychiatry and Behavioral Science, State University of New York at Stony Brook, Stony Brook, New York, United States of America, 4 AIDS and Cancer Viruses Program, SAIC-Frederick/NCI-Frederick, Frederick, Maryland, United States of America Abstract Background: BORIS/CTCFL is a paralogue of CTCF, the major epigenetic regulator of vertebrate genomes. BORIS is normally expressed only in germ cells but is aberrantly activated in numerous cancers. While recent studies demonstrated that BORIS is a transcriptional activator of testis-specific genes, little is generally known about its biological and molecular functions. Methodology/Principal Findings: Here we show that BORIS is expressed as 23 isoforms in germline and cancer cells. The isoforms are comprised of alternative N- and C-termini combined with varying numbers of zinc fingers (ZF) in the DNA binding domain.