XIAP、OMI%2fHTRA2蛋白对HeLa细胞影响以及两者间相互作用实验的研究.pdf

波下记录到部分细胞的红色荧光，当作为受体的DsRed2一XIAP荧光漂白后作 为弥漫分布在细胞核和细胞浆。(2)米非司酮能促进Hela细胞凋亡； 治疗宫颈癌的一个新靶点，米非司酮促HeLa细胞凋亡的作用机制可能与 与Omi／HtrA2在部分细胞中存在相互作用。 关键词：宫颈癌细胞系(HeLa)；凋亡；米非司酮；XIAP；Omi／HtrA2； 流式细胞术；荧光共振能量转移 4 A2对IteLa细胞的影响以技两行间的相且作用 遵义医学院硕I{Uf究生毕业论义XIAP．0mi／ht Theeffectsoffluorescentrecombinant protein XIAP HeLacells with and on plasmid Omi／HtrA2 of isoneofhallmarksof is Abstract：bjective：Failureapoptosis cancer,and tocontributeboth andtumor isan widelythought tumorigenesisprogression．Then，it toresearchthefundationoflAPsfor most importantaspect neoplasm．XIAP,the whichis IAPsis inhibitor oneof believedtoinhibite other apopotosiseventuallyby downstream as isanovel activators，such caspase一3，6，7，9．’Omi／HtrA2pro—apoptotic able advance and inhibitorof is to by gene，which apoptosisbindinginhibiting inhibit its may properenzymatic apoptosisprotein(tar)，whichCaspases，and effectionofXIAP onHeLacellswas activity．Besides，the and：Omi／HtrA2 reported aimofthis wasto the and seldom．Therefore，the very study investigateexpression functionofXIAPandOmi／HtrA2inHeLa their to