30759-762接665食品安全与检测4-12固相萃取-高效液相色谱法测定饮料中维生素B12.pdfVIP

现代食品科技 Modern Food Science and Technology 2010, Vol.26, No.7 固相萃取-高效液相色谱法测定饮料中维生素B12 1,3 1,3 2,3 1,3 2,3 周芳 ，蔡双福 ，文灿 ，马思娜 ，许梅花 （1.广东省食品质量监督检验站，广东广州 510308 ）（2.广东省食品工业研究所，广东广州 510308 ） （3.广东省食品工业公共实验室，广东广州 510308 ） 摘要：利用Agela PCX 固相萃取柱对饮料中的维生素B12进行浓缩和净化，建立了测定饮料中维生素B12的固相萃取－高效液相色谱法。方法的线 性范围为0.1~20.00 μg/mL，相关系数为0.99996。方法检出限为0.2 ng/mL 。三个浓度水平的加标回收率分别为78.7% 、83.0%、83.3%，相对标准偏差小于3%。 关键词：高效液相色谱法；固相萃取；维生素B12；饮料 文章篇号：1673-9078(2010)7-759-762 Determination of Vitamin B12 in Beverages using Solid-phase Extraction-high Performance Liquid Chromatography ZHOU Fang-mei1,3, CAI Shuang-fu1,3, WEN Can2,3, MA Si-na1,3, XU Mei-hua2,3 (1.Guangdong Food Quality Supervision and Inspection Station, Guangzhou 510308, China) (2.Guangdong Food Industry Institute, Guangzhou 510308, China) (3.Guangdong Provincial Food Industry Public Laboratory, Guangzhou 510308, China) Abstract: A method for the determination of vitamin B12 in beverages using solid-phase extraction-high performance liquid chromatography was developed. The Agela PCX solid-phase extraction column was used to extract and purification of vitamin B12 in the sample. The calibration curve of vitamin B12 showed good linearity in the range of 0.1~20.00 μg/mL with correlation coefficient of 0.99996. The determination limit was 0.2 ng/mL. And the average recoveries at three levels were 78.7%, 83.0 %, and 83.3%. The relative standard deviations were lower than 3%. Key words: high perform liquid chromatography (HPLC); solid-phase extraction (SPE);vitamin B12; beverages 759 维生素B12又称钴胺素，是一种水溶性维 本方法用丙酮沉淀浓缩后饮料中的蛋白 生素，也是唯一含有主要矿物质的维生素。一 质和糖份，用石油醚来去除饮料中的脂肪，再