应用荧光原位杂交法检测乳腺癌石腊样本中HER-2基因扩增.docVIP

应用荧光原位杂交法检测乳腺癌石腊样本中HER-2基因扩增 　【摘要】 目的 探讨荧光原位杂交法（Fluorescence in situ hybridization, FISH）检测乳腺癌HER2基因扩增在临床病理诊断及分子靶向治疗中应用的可能性。方法 用FISH技术和免疫组化（Immunohistochemistry，IHC）技术检测50例乳腺导管癌石蜡包埋标本并比较两种方法的结果以及与临床病理的关系。结果 16/50例HER2蛋白表达阳性，其中强阳性5例，中度阳性9例，弱阳性2例；11/50（22%）例乳腺癌标本FISH技术检测HER2基因扩增阳性，其中5/5为免疫组化HER2蛋白强阳性病例；6/9为中度阳性病例，其中1例为17号染色体多倍体与HER2基因扩增。HER2基因扩增与蛋白表达与乳腺癌转移有关（Plt;0.05）。结论 FISH技术可稳定地检测用IHC确定的HER2蛋白阳性乳腺癌中HER2基因的扩增状况，并用于临床赫赛汀分子靶向治疗病例的筛选。 【关键词】 HER2/癌基因；荧光原位杂交；乳腺癌 　　Application of Fluorescent in Situ Hybridization (FISH) to Detect the Amplification of HER2 Gene in Paraffin Sample of Breast Cancer 　　Abstract：Objective To explore the possibility of application with the method to detect amplification of HER2 gene by fluorescence in situ hybridization (FISH) in clinical pathology and molecular targeting therapy in breast cancer, and investigate the relationship between HER2 gene amplification and clinicopathological data.Methods Detecting 50 cases of formalinfixed and paraffinembeded breast ductal carcinoma samples compared with result of immunohistochemistry and then analyzed with clinicopathological data.Results Sixteen of fifty (32.0%) cases of breast carcinoma presented positive for HER2 protein expression, including 5 strong, 9 moderate and 2 weak expressions, respectively. Eleven of fifty （22.0%）cases of breast cancer showed positive for HER2 gene amplification, in which 5/5 was the cases showing strongly positive and 6/9 presenting moderately positive expression for HER2 protein by immunohistochemistry. And the one with both amplification of HER2 gene and polysomy of chromosome 17 was also moderately positive expression for the protein. Both gene amplification and protein expression of HER2 were corresponding to metastasis of lymph nodes (Plt;0.05).Conclusion FISH technique can stably detect the amplification of HER2 gene especially for the positive cases for HER2 protein and it is clinically useful to select the cases for molecular targeting thera