弓形虫感染对小鼠卵巢功能影响.docVIP

弓形虫感染对小鼠卵巢功能影响 　【关键词】 弓形虫；卵巢疾病 　　【摘要】 目的 研究弓形虫感染对卵巢功能的影响。方法 将雌性小鼠随机分为实验组和对照组，每组各30例，实验组在雌性小鼠感染弓形虫后第45、90天的发情间期采血，对照组也在相应的时期采血。采用放射免疫法测定血清中雌二醇（E2）含量，并于完成两次采血后取两组小鼠卵巢和脑组织送病理检查。结果 实验组45天时血清E2为（12.37±5.56）pg/ml，90天时为（7.98±4.59）pg/ml；对照组45天时血清E2为（10.99±8.71）pg/ml，90天为（10.85±5.06）pg/ml；实验组90天时E2水平下降，与45天时及对照组比较，差异均有显著性（P＜0.05）。实验组小鼠脑组织见慢性炎症改变及弓形虫包囊体；卵巢呈炎性改变；对照组小鼠脑组织和卵巢未见异常。结论 弓形虫感染可以导致小鼠卵巢功能下降。 【关键词】 弓形虫；卵巢疾病 Effects of toxoplasma infection on ovarian function in mice 【Abstract】 Objective To study the effects of toxoplasma infection on ovarian function in Kunming female mice.Methods The Kunming female mice were devided to two groups （each n=30）:experiment group were infected with AH strain of toxoplasma,and control group were not infected with AH strain of toxoplasma.After 45 days and 90 days,the blood were collected in the estrum interphase,and estrogen in serum were detected by radioimmunoassay.The histology of the brain and ovary were observed by microscope.Results The level of estrogen in serum of experiment group was （12.37±5.56）pg/ml at 45 days,and （7.98±4.59）pg/ml at 90 days;The level of estrogen in serum of not infected group was （10.99±8.71）pg/ml at 45 days,and （10.85±5.06）pg/ml at 90 days.The level of estrogen in serum of experiment group at 90 days was reduced significantly compared with the level at 45 days and control group（P＜0.05）.The histology of the brain was phlogistic change and found Toxoplasma cysts body,and histology of the ovary was phlogistic change in experiment group;the histology of the brain and ovary in control group was not found abnormal.Conclusion The infection of toxoplasma possibly reduced the ovarian function in KM female mice. 【Key words】 toxoplasma;ovary disease 弓形虫是一种机会性致病的寄生虫，对人类的危害日益受到重视。尤其是对孕妇、艾滋病患者、器官移植使用免疫抑制剂等部分人群。弓形虫可寄生于成熟的红细胞以外的所有有核细胞，引起多器官的损害，如脑、心、肺、脾、肾、肌肉等。本实验通过雌性昆明小鼠感染弓形虫AH株后检测其雌激素的水平，并对小鼠的卵巢、脑组织行病理检查，研究弓形虫对卵巢功能的影响。现将结果报告如下。 1 材料与方法 1.1 实验动物与虫株来源 实验动物为SPF级雌性昆明种小鼠，体重30~37g，性成熟，共60只，均由湖北省疾病预防控制中心医学实验动物室提供。动物饲养温度为17℃~24℃，