心肌缺血预处理对未成熟心肌细胞凋亡影响.docVIP

心肌缺血预处理对未成熟心肌细胞凋亡影响 　 作者：孙忠东，池一凡，杨铁南，侯文明，牛兆倬，唐子龙 【关键词】 缺血预处理 摘要：目的 探讨心肌缺血预处理(MIP)对未成熟心肌bcl-2、bax、fas蛋白表达的影响。方法 采用兔Langendorff模型，24只幼兔随机分为3组，对照组(C,n=8)：仅灌注KH液180min，然后取标本；缺血/再灌注组（I/R, n=8）：灌注20min后，停灌45min，复灌120min；心肌缺血预处理组(MIP, n=8)，灌注20min，反复2次停灌5min，复灌5min，然后重复I/R组缺血/再灌方法。以凋亡细胞原位标记、琼脂糖凝胶电泳检测DNA片段梯及bcl-2、bax、fas蛋白表达作为检查指标。结果 琼脂糖凝胶电泳检测DNA片段梯：MIP组与I/R组比较，光密度明显减弱； MIP组与I/R组比较，bcl-2表达明显增多，bax、fas表达明显减少。结论 MIP通过影响bcl-2、bax、fas基因蛋白的表达减少心肌细胞凋亡。 　　关键词： 缺血预处理；心肌；细胞凋亡；基因蛋白 　　The Effects of Myocardial Ischemic Preconditioning on Immature Myocardial Cell Apoptosis 　　　Abstract: OBJECTIVE To investigate the effects of myocardial ischemic preconditioning (MIP) on immature myocardial cell apoptosis and expression of bcl-2,bax,fas protein .METHODS Isolated working rabbit heart model was used in this study, 24 rabbits (aged 14～21 days) were divided into 3 groups (n=8) . Control group was only perfused with Krebs-Henseleit (KH) 180 minutes. Ischemia/reperfusion(I/R) group was perfused 20 minutes ,and then kept under ischemia 45 minutes, followed by reperfustion 120 minutes. Myocardial ischemic preconditioning (MIP) group was perfused 20 minutes ,and then repeatly ischemia 5 minutes followed reperfusion 5 minutes 2 times,finnaly reperfused 120 minutes. Such indexes, including the TUNEL, nucleosomal ladder of DNA fragments, and the expression of bcl-2, bax,and fas gene were detected.RESULTS MIP group there was reduction in TUNEL cell apoptosis, ladder of DNA fragments,expression of bax and fas gene,and increase in expression of bcl-2 gene.CONCLUSION This study demonstrated that MIP decrease immature myocardial cell apoptosis by affecting the expression of bcl-2, bax,and fas gene . 　　Key words：Ischemic preconditioning;Myocardial; Apoptosis;Gene proteins 　　细胞凋亡(apoptosis)是指有核的细胞在一定条件下通过启动自身的机制而发生的一种自然死亡过程。细胞凋亡广泛参与机体的正常发育和各种病理生理过程。细胞凋亡发生不足或凋亡过度均可以导致机体发生多种病理生理变化。在成熟心肌实验表明MIP可减少心肌细胞凋亡的发生，其中bcl-2、bax、fas等基因蛋白的表达在细胞凋亡中起重要作用。MIP对未成熟心肌细胞凋亡有否影响，尚未可知。本实验目的观察MIP对未成熟心肌细胞凋亡和bcl-2、bax、fas等基因蛋白的表达的影