法国研究高压氢的免疫学效应.pdfVIP

C.R. Acad. Sci. Paris, Sciences de la vie / Life Sciences 324 (2001) 719–724 © 2001 Académie des sciences/Éditions scientifiques et médicales Elsevier SAS. Tous droits réservés S0764446901013506/FLA Sciences médicales / Medical sciences Anti-inflammatory properties of molecular hydrogen: investigation on parasite-induced liver inflammation a a a b c Bouchra Gharib , Stéphane Hanna , Ould M.S. Abdallahi , Hubert Lepidi , Bernard Gardette , Max De Reggia* a U399 Inserm, université de la Méditerranée, 27, boulevard Jean-Moulin, 13005 Marseille, France b Laboratoire d’histologie, université de la Méditerranée, 27, boulevard Jean-Moulin, 13005 Marseille, France c Comex SA, 36, boulevard des océans,13009 Marseille, France Received 12 January 2001; accepted 25 April 2001 Communicated by Jean Rosa Abstract – Molecular hydrogen reacts with the hydroxyl radical, a highly cytotoxic species produced in inflamed tissues. It has been suggested therefore to use gaseous hydrogen in a new anti-inflammatory strategy. We tested this idea, with the aid of the equipment and skills of COMEX SA in Marseille, a group who experiments with oxygen–hydrogen breathing mixtures for professional deep-sea diving. The model used was schistosomiasis-associated chronic liver inflammation. Infected animals stayed 2 weeks in an hyperbaric chamber in a normal atmosphere supplemented with 0.7 MPa hydrogen. The treatment had significant protective effects towards liver injury, namely decreased fibrosis, improvement of hemodynamics, increased NOSII activity, increased antioxidant enzyme activity, decreased lipid peroxide levels and decreased circulating TNF-α levels. Under the same conditions, helium exerted also some protective effects, indicating that hydroxyl radical scavenging is not the only protective mechanism. These findings indicate tha