姜黄素对人肝L-02细胞胆固醇合成及转运蛋白表达影响.pdfVIP

observe the formation of intracellular lipid droplets under oil red O dye.Detect the intracellular amount of TC and CE and calculate the ratio of intracellular CE to TC. Then detect the HMG-CoA reductase and caveolin-1 mRNA’s expression of the fatty changed hepatocytes, determine the best duration that curcumin decreases intracellular CE, and draw out the duration-effect relation curve. Using ±s to represent experimental data, using SPSS13.0 statistical software to analyze the data, a P value of 0.05 is considered significantly important. Results: After exposing normal hepatocytes to 50% fetal bovine serum for 24 hours ,find out numerous lipid droplets in the luminal side of the cells ,which can be dyed to red with oil red O dye. what ’s more, intracellure CE increases dramatically through measurement of HPLC. Thus,fatty changed hepatocytes can be set up successfully.Then exposing the fatty changed hepatocytes to different concentrations of curcumin for 24 hours. The result displays: oil red O dye shows, the intracellular lipid droplets are much less than before exposure. Curcumin decreases the intracellular TC and CE of the fatty changed liver cells,while increase the amount of FC in the medium as well concentrationally dependently.What ’s more,it decreases the HMG-CoA reductase mRNA expression and increases the caveolin-1 mRNA expression concentrationally dependently at the same time; Then After mixing the curcumin of 100umol/l with the fatty changed hepatocytes for 0, 6, 12, 24hours, the result says: the decrease of TC and CE in the cells and increase of FC in the medium by curcumin is dependent on the duration , at the same time the decrease of HMG-CoA reductase and increase of caveolin-1 mRNA’s