重庆地区博尔纳病病毒种系发生分析.docVIP

重庆地区博尔纳病病毒种系发生分析 张英英展群岭徐鸣明余建萍翟红刘妍汐陈晓彭丹朱丹 谢鹏(重庆医科大学：1附属第一医院神经内科， 2神经科学研究中心，重庆 400016) 摘 要纳病病毒(Borna disease virusBDV)基因特征及其种系发生。方法　采用逆转录聚合酶链反应luorescence quantitative nested RT-PCR，FQ-nRT-PCR），外周血单核细胞(peripheral blood mononuclear cellsPBMCs)BDV p24片段，检出阳性序列和前期检出的5例重庆地区山羊和神经精神患者阳性BDV p24基因序列，与GenBank中5个国家7个动物种属33例BDV p24基因序列进行比对，分析其核苷酸和氨基酸序列同源性，重建基因系统发生树。结果　Strain V、H1766及He/80比对，9例核苷酸和氨基酸序列同源相似性分别为94%～100%和82%～100%；其余1例为95%～98%和89%～93%。结论　可能存在BDV独立株和源于外来疫病病原传入的流行株。 关键词博尔纳病病毒； 中图法分类号S852.65+9.5 文献标识码：Ahylogenetic analysis of Borna disease virus in Chongqing ZHANG Ying-ying1，ZHAN Qun-ling1，XU Ming-ming1，YU Jian-ping1，ZHAI Hong1，LIU Yan-xi1，CHEN Xiao1，PENG Dan1，ZHU Dan1，XIE Peng1，2（1Department of Neurology，the First Affiliated Hospital，2Neuroscience Research Center，Chongqing Medical University，Chongqing 400016，China) Abstract: Objective To gain detailed insights into the genetic characters and phylogeny of Borna disease virus in Chongqing, China. Methods BDV p24 segment were detected by fluorescence quantitative nested RT-PCR from peripheral blood mononuclear cells of fifty healthy cattle, fifty healthy goats, fifty healthy pigs and twenty patients with viral encephalitis. Phylogenetic analysis of BDV p24 positive samples were performed by alignment with five positive gene sequences of healty goats and patients with neuropsychiatry in preliminary studies in Chongqing and thirty-three sequences of seven animals in five countries from GeneBank. The sequence homologous similarity of the nucleotide and the amino acid sequences was analyzed and gene phylogenetic tree was reconstructed. Results One part of ten nucleotide sequences formed Chongqing independent branch and the other clustered within Germany - Switzerland - Austria - Japan - Chongqing mixed branch. There was high identity within the branch. Aligned with Strain V, H1766 and He/80, the homologous similarity in nine sequences of both the nucleotide and the amino acid was from 94% to 100% and 82% to 100%, and in