人股骨头制作的dbm和dpb的成骨诱导活性体外研究张波柳峰沈伟伟.docVIP

人股骨头制作的DBM和DPB的成骨诱导活性体外研究 张波 柳峰 沈伟伟 赵建华 第三军医大学大坪医院野战外科研究所骨科 重庆 400042 摘要：目的 研究人无菌性坏死股骨头制作的脱钙骨基质（demineralized bone matrix，DBM）（）3 mm×3 mm×5 mm大小的骨粒并制作成DBM和DPB，然后与人骨髓间充质干细胞（human mesenchymal stem cells，hBMSCs）联合培养21天，并以单层细胞培养为对照组。在1、3、5、7、14天检测细胞在材料上的增殖活性；在1、7、14、21天检测培养基中的碱性磷酸酶（alkaline phosphatase）（）（）（0.01）（0.05）The osteogenic activity of DBM and DPB made from human femoral head:an in vitro study ZHANG bo,ZHAO jian-hua(Department of Orthopaedics, Daping Hospital, Institute of surgery research,Chongqing,400042,China) Abstract: Objective To observe the osteogenic activity of demineralized bone matrix（DBM） and deprotenized bone（DPB） made from aseptic necrosis of femoral head. Methods The femoral head was cut into pieces of the size 3mm×3mm×5mm, which were made into DBM and DPB.These two kinds of biomaterials were seeded with human bone mesenchymal stem cells（hBMSCs）. Monolayer Cells without biomaterials were cultivated as control.The cell proliferation activity on biomaterials _________________ 作者简介：张波,四川南充市人,硕士研究生,电话:（023 E-mail:challenger163.com 通信作者：赵建华，教授，硕士生导师，电话：（023 研究方向：脊柱外科与组织工程 E-mail：zhaojianhua1964@ was evaluated on day 1，3，5，7. The concentration of alkaline phosphataseALP）, osteocalcin ( OC)、Ca2+ w evaluated on day 1，3，7，14，21. ResultsThese two kinds of biomaterials had signifcant cell proliferaton activity compared with monolayer cells （ P 0.05）, The DBM group performed more active than others. These two kinds of biomaterials also had osteogenic activity，The concentration of ALP, OC, Ca2+ of DBM group were signifcant higher than DPB group and monolayer cells ( P 0.05. Conclusion DBM and DPB made from aceptic necrotic femoral head have osteogenic activity, espeicially DBM. Key words: aseptic necrosis of femoral head; demineralized bone matrix; deproteinized bone;human bone mesenchymal stem cells 股骨头无菌性坏死是骨科常见的一种髋关节疾病，绝大部分患者最后通过髋关节置换术以减轻疼痛和改善患肢功能，因此产生了大量的被置换下来的股骨头。但目前对无菌性坏死股骨头用来制作骨生物支架材料尚未引起重视，临床置换下来的股骨头部分被废弃，部分予以简单的消