大黄素改善脂多糖引起的胰岛素抵抗机制研究.pdfVIP

·71 · 2010 44 8 SH..JTCM Aug., 2010;Vo.l44 No.8 　　　　　　　　　　　　　　　　　　　 , 　 (　 200 025 ) 【　】　目的　 (emodin) (LPS)。 方法　 C2 C12 , , ;, RT-PCR mRNA, (MAP ) 1/2 (ER 1/2 )ER Phos- phor-ER , AMP ACC。 结果　 ① IL-6、 TNF-αmRNA;② MAP ER ;③ C2 C12 AMP ACC;④ C2C12 。 结论　 LPS MAP ER , IL-6、 TNF-α; C2 C12 , AMP ACC, , 。 【】　;;; 【】　 R285.5　　【】　A　　【】　100 7-13 34 (20 10 )08-0 071-04 Molecularmechanismsofemodinininhibitinginsulinresistanceinducedbylipopolysacchide ZHANGRong　ZHURong-feng　 XU Jun　 ZHOULi-bin　ZHANGD　iYANG Jian　YANGYing　CHENMing-dao ShanghaiInstituteofEndocrineandMetabolicDisease,sShanghaiClinicalCenterforEndocrineandMetabolicDiseases Abstrac:t Objective　 Toexplorethemechanismsofemodinintheinhibitionofinsulinresistanceinducedbylipopolysacchide(LPS)inC2 C12 myotube.sMethods　 C2C12 myotubeswereinducedunderstandarddifferentiationprocessandpreincubatedwithLPSatvarioustimeforaninsulinresistancemode,landthenincubated withemodinatdifferentconcentration.sThemRNA expressionsofIL-6 andTNF-αwereanalyzedwithquantitativerealtmie-PCR; theeffectsofemodinonMAP signalproteinactivitieswereinvestigatedwithmimunoblottinganalysisofphosphorylatedER, andtheeffectsofemodinonAMP signalproteinactivitieswerein- vestigatedwithmimunoblottinganalysisofphosphorylatedAMP andACC.Results　① EmodininhibitedthemRNAexpressionsofIL-6 andTNF-α inadose-de- pendentmanne.r② EmodininhibitedtheproteinphosphorylationlevelsofER.③ EmodinreducedoxgenconsumptioninC2 C12 myotube.s④ Emdoinactivated thephosphoreylationofAMP andACCinC2 C12 myotube.s Conclusion　 EmodincaninhibitthemRNAexpressionsofinflammatoryfactorsinducedbyLPSand inhibittheactivityofER, andactivatethephosphoreylationofAMP andACC tomiprovetheinsulinresistanc.e eywords: Emodin; insulinresistance;C2C12 myotub;e lipopolysacchide(LPS) 　　、 2 、 , , [ 3] , 。 。 2 (