澳洲青苹多酚氧化酶特性的研究.pdfVIP

DO I :10.15933/j .cnki .1004 -3268.2006.09.035 河南农业科学 1 1 1 1 2 李佩艳,侯玉泽 ,李松彪, 刘建学 ,仇农学 (1., 47 1003;2., 7 10062 ) :以邻苯二酚为底物, 对澳洲青苹中的多酚氧化酶进行了探讨。分别研究了pH 值、温度、底物 度及抑制剂等因素对多酚氧化酶活性的影响,结果表明:澳洲青苹多酚氧化酶最适pH 值为6.5; 最适温度为35 ℃;90 ℃处理3 min, 多酚氧化酶活性基本被抑制;添加 度为125 mg/kg 抗坏血酸 或者 度为600 mg/kg 的柠檬酸或者 度为50 mg/kg 的亚硫酸氢钠后, 多酚氧化酶活性分别为 对照的36%, 38%和23%。澳洲青萍多酚氧化酶的米氏方程参数为:Km=0.137 mol/L, V max= 0.460 U/min。 :澳洲青苹;多酚氧化酶;酶促褐变 :S661.1 　　:A 　　:1004-326 8(2006)09-0093-04 Charac eris ics of Polyphenol Oxidase in Granny Smi h A pple 1 1 1 1 2 L I Pei-yan , HOU Yu-ze , LI Song-biao , LI U Jian-x ue , QIU Nong-x ue (1.College of Food and Bioengineering, Henan Universi y of Science and Technology, Luoyang 47 1003, China; 2.F ood Engineering Depar men of Shaanxi N ormal U niversi y, Xian 7 10062, China) Abstract:T he op imum pH , op imum empera ure, subs ra e concen ra ion and he inhibi o rs of polyphenol oxidase f rom Granny Smi h were analy zed w i h he ca echol subs ra e.T he resul s show ed ha he op imal pH and empera ure w ere 6.5 and 35 ℃, respec ively .Once he empera ure was over 60 ℃,PPO became inac ive quickly .T he PPO w as almos inac iva ed a 90 ℃fo r 3 min .PPOs M is- cherlisch Equa ion parame ers Km=0 .137 mol/L and V max =0.460 U/min .A dding 125 mg/kg V c o r 600 mg/kg ci ric acid o r 50 mg/kg N aHSO3 in reac ion sys ems, he ac ivi y of PPO decreased o 36%, 38 %and 23%, respec ively , compared w i h he check. Key words:Granny Smi h ;Polyphenol oxidase;Enzyma ic brow ning 　　(Polyphenol , 。 o xidase, PPO, EC ) 5.4 %～7 .0%, [ 1] , 2.0%, 1.0%～1.5 %。, , , 。, 、, 。