水稻稻瘟病菌诱导表达启动子osq16p的克隆与功能分析-作物学报.pdfVIP

作物学报 ACTA AGRONOMICA SINICA 2012, 38(6): 980987 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2012.00980 水稻稻瘟病菌诱导表达启动子OsQ16p 的克隆与功能分析 王 光 吴智丹 张 磊 刘凤权 邵 敏* / , 210095 : qRT-PCR , OsQ16 PCR 5′1 229 bp , OsQ16ppBI121 gus CaMV35S , pBIQ16p, , GUS qRT-PCR : gus ; 12 h, GUS 2.7 ; (salicylic acid, SA)(methyl jasmonate, MeJA) 12 h, GUS 3.1 3.5 , OsQ16p , MeJA SA : ; ; OsQ16p; GUS; Cloning and Functional Analysis of Magnaporthe oryzae-Induced Promoter OsQ16p in Rice * WANG Guang, WU Zhi-Dan, ZHANG Lei, LIU Feng-Quan, and SHAO Min College of Plant Protection, Nanjing Agricultural University / Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, China Abstract: The qRT-PCR analysis showed that, in Nipponbare (Oryza sativa L. ssp japonica ), the expression of OsQ16 gene was induced by Magnaporthe grisea . The 1 299 bp-fragment of 5-end of OsQ16 gene, named as OsQ16p, was amplified by PCR from Nipponbare. The plasmids pBIQ16p was constructed by replacing the CaMV35S promoter of pBI121 with the OsQ16p, and transformed into Nipponbare through Agrobacterium -mediated transformation. The analysis of GUS activity and qRT-PCR showed that gus gene could express in transgenic plants and calli. The expression of gus gene in transgenic plants was obviously enhanced by M. grisea . After treatment with the resistance-related signaling molecules SA and MeJA, the GUS activities in trans- genic plants were increased by 3.1- and 3.5-fold, respectively. It suggested that M. grisea , SA,