水菖蒲内生菌分离与抗菌活性菌株筛选.pdfVIP

※生物工程 食品科学 2010, Vol. 31, No. 09 211 1 1, 1,2 1 1 周晓坤 ，陈 钧 * ，韩邦兴 ，何 佳 ，刘长风 (1.江苏大学药学院，江苏镇江 212013 ；2.植物细胞工程安徽省工程技术研究中心，安徽六安 237012) ：对水菖蒲内生真菌进行分离，并进行抗菌活性的筛选。采用改良氯化三苯四氮唑显色(TTC)微量稀释法 筛选抗菌活性菌株。以18 种常见导致食品腐败的细菌、酵母菌和霉菌为指示菌研究活性菌株的抗菌能力。结果显 示，水菖蒲内存在广泛的具有抗菌活性的内生菌，占内生菌总数的40.7% ，高活性菌株占内生菌总数的12.39%。 其中S9 菌株发酵液具有抗细菌、酵母菌和丝状真菌的广谱抗菌能力，是潜在的食品防腐抗菌资源。 水菖蒲；内生菌；抗菌活性 Screening and Inhibitory Activity of Endophytic Fungi from Acorus calamus L. 1 1, 1,2 1 1 ZHOU Xiao-kun ，CHEN Jun *，HAN Bang-xing ，HE Jia ，LIU Chang-feng (1.College of Pharmacy, Jiangsu University, Zhenjiang 212013, China；2. Anhui Engineering Technology Research Center of Plant Cell Engineering, Lu ’an 237012, China) Abstract ：In order to isolate and screen endophytic fungi with inhibitory activity from Acorus calamus L., active strains were screened through modified TTC micro-dilution method. MIC (minimum inhibitory concentration) and MBC (minimum bacte- ricidal concentration) were detected using 18 food spoilage strains. Results indicated that 40.7% endophytes exhibited anti- microbial activity and 12.39% endophytes exhibited higher antimicrobial activity. In addition, strain S9 exhibited the broad- spectrum antimicrobial activity to bacteria, yeast and mycelia fungi. Active endophytic strains from A. calamus L. are potential resources for food preservation. Key words ：Acorus calamus L. ；endophytic fungi ；antibacterial activity 中图分类号： Q933 文献标识码：A 文章编号：1002-6630(2010)09-0211-05 植物内生菌是指那些在其生活史的一定阶段或全部 1 材料与方法 阶段生活在健康植物组织或器官内部的并不引起宿主