水稻纹枯病生防内生菌糖蜜草固氮螺菌的分离与鉴定-植物病理学报.pdfVIP

植物病理学报 ACTA PHYTOPATHOLOGICA SINICA　 42(4):425-430(2012) 水稻纹枯病生防内生菌糖蜜草固氮螺菌的分离与鉴定 王兰英, 谢 颖, 廖凤仙, 王 琼, 骆焱平* (海南大学热带作物种质资源保护与开发利用教育部重点实验室,海南大学环境与植物保护学院, 海口570228) 摘要:本文首先对砂仁内生细菌进行分离,以水稻纹枯病菌(Rhizoctonia solani)为靶标菌对获得菌株进行离体拮抗活性、盆 栽及田间试验测定。 结果表明:获得的27株内生细菌中有4株具有较好的离体抑菌活性,其中SRJ2-4抑菌效果最好,抑菌 带达到18 mm;SRJ2-4 的盆栽防效及田间防效分别为80.7%与79.4%,与其它菌株相比达极显著水平。 SRJ2-4 处理的亩 产量为488.79 kg,高于其他药剂处理。 对该菌株形态、生理生化及16S rDNA序列进行分析,将该内生菌鉴定为糖蜜草固 氮螺菌(Azospirillum melinis)。 关键词:砂仁;内生细菌;水稻纹枯病菌;糖蜜草固氮螺菌 Isolation and identification of an endophytic bacteria Azospirillum melinis against Rhizoctonia solani　 WANG Lan-ying,XIE Ying,LIAO Feng-xian,WANG Qiong,LUO Yan-ping (Key Laboratory of Protection and Development Utilization of Tropical Crop Germplasm Resources,Ministry of Education, College of Environment and Plant Protection,Hainan University,Haikou 570228,China) Abstract: Twenty-seven endophytic bacterial strains isolated from Fructus amomi were tested for in vitro antagonistic activity,pot experimentandfieldtrialagainsttherice sheathblightpathogen,Rhizoctoniasolani. The resultsrevealed that 4 of the 27 isolates showed good anti-fungal activity against Rhizoctonia solani,of which SRJ2-4 exhibited the best effect with an inhibition zone up to 18 mm in diameter. The control efficacy of SRJ2-4 strain in pot experiment and field trial were 80.7% and 79.4% respectively,significantly higher than those of other isolates. The yield ofthe fieldtreated with SRJ2-4 wasalsofoundtobethehighest,up to -2 488.79 kg ·667 m . According to the morphological analysis,physiological and biochemical determination and 16SrDNA sequence comparison,the strain SRJ2-4 wasidentified asAzospirillum melinis. Key words: Fructus amomi;endophytic bacteria;Rhizoctonia solani;Azospirillum melinis 中图分类号: S476　 　 　 　 　 文献标识码: A　 　 　 　 　 文章编号:0412-0914(2012)04-0425-06 　 　 水稻纹枯病(Rhizoctonia solani)又称“花秆”、 了水稻纹枯病的生防研究工作。 为了寻找和发现高 “烂脚病”、“富贵病”,分布于亚洲、非洲和美