响应面法优化银翘天甘含药血清制备工艺-中国农学通报.pdfVIP

中国农学通报 2012,28(17):75-81 Chinese Agricultural Science Bulletin 响应面法优化银翘天甘含药血清制备工艺 王宏军，周铁忠，王术德，李 丽，刘英姿 （辽宁医学院，辽宁锦州121001） 摘 要：为了优化银翘天甘含药血清的制备工艺。在单因素试验的基础上，选取采血时间、给药次数及给 药浓度为自变量，以小鼠血清中绿原酸和连翘苷含量的综合评分为响应值，采用响应面分析方法，研究 各自变量及其交互作用对综合评分的影响。利用Design-expert 软件分析得到二次多项式回归方程的预 测模型，并确定了银翘天甘含药血清最佳制备工艺条件为：采血时间30 min 、给药次数4 次、给药浓度 0.02 g/mL ，理论计算综合评分为95.62% ，小鼠血清中绿原酸浓度为33.65 µg/mL，连翘苷浓度为 193.86 µg/mL。该研究为银翘天甘含药血清的制备奠定了基础。 关键词：中药；含药血清；绿原酸；连翘苷；响应面分析 中图分类号：S853.74 ，R965.1 文献标志码：A 论文编号：2011-2935 OptimizationofPreparationTechnologyofMedicatedSerumofYinqiaotiangan inMicebyResponseSurfaceMethodology Wang Hongjun, Zhou Tiezhong, Wang Shude, Li Li, Liu Yingzi (LiaoningMedicalUniversity, Jinzhou Liaoning 121001) Abstract: In order to optimize preparation technology on medicated serum of Yinqiaotiangan in mice, on the base of single factor investigation, taking-blood time, administration times, administration concentration were selected for the independent variable and comprehensive score on the concentration of chlorogenic acid and forsythia in the mice serum was as response value. Effects of every independent variable as well as their interactions on response value were studied by response surface analysis. The predictive model of two polynomial regression equations was analyzed by Design-expert software. The results showed that preparation technology was concluded as take blood time 30 min, administration times 4 times and administration concentration 0.02 g/mL. In theoretical calculation, comprehensive score was 95.62%. In this condition, the concentration of chlorogenic acid