mTOR及S6K1在免疫介导再生障碍性贫血模型小鼠骨髓中的表达.pdfVIP

()2017 1 11 2 Chin J Clinicians(Electronic Edition),January 15,2017,Vol.11,No.2 255 mTOR S6K1 张晓东 张伟华 葛晓静 mTORS6K1 12 3mTOR S6K1 SPSS 22.0 t Pearson 1 5 14 d 5 WBCHbPLT 9 14 d 10.520.7310 /L1326.3g/L469114 9 9 9 10 /L0.970.5710 /L749.3g/L573210 /LP 0.001 2mTOR S6K1 74.846.3363.455.38 113.2716.23108.5612.77P 0.001 3mTOR S6K1 WBCHbPLT mTOR S6K1 mTOR S6K1 mTOR S6K1 Expression of mTOR and S6K1 in bone marrow of immune conductive aplastic anemia mice Zhang Xiaodong, Zhang Weihua, Ge Xiaojing. Department of Hematology, the First Affiliated Hospital, Shanxi Medical University, Taiyuan 030001, China Corresponding author: Zhang Weihua, Email: ZWHLZH@126.com Abstract Objective To research the mechanism of bone marrow fat of aplastic anemia mice, through detecting the expression of mTOR, a fat regulatory factor, and S6K1, which is located in mTOR pathway downstream. Methods (1) The success of immune conductive aplastic anemia mice was the key factor of the whole experiment. (2) Observed the growing status of the mice, and measured the hemogram regularly to verify the success of aplastic anemia mice model. (3) Detected the expression of mTOR and S6K1 in bone marrow of immune conductive aplastic anemia mice with immunohistochemistry. All data was checked with software SPSS 22.0. To check up the data of all groups with normal test. t-test of single sample was used in the comparison of means of two groups. Correlation analysis was done with Pearson correlation. Results (1) The AA mice model was succes