酸性木聚糖酶产生菌株的筛选和酶学性质分析-中国农业科学.pdfVIP

中国农业科学 2010,43(7):1524-1530 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2010.07.026 酸性木聚糖酶产生菌株的筛选和酶学性质分析 国春艳，刁其玉，乔 宇，屠 焰，张乃锋，姜成钢 （中国农业科学院饲料研究所，北京 100081 ） 摘要：【目的】从自然环境筛选出可用于饲料工业加工用途的酸性木聚糖酶产生菌株。【方法】通过富集培养 定向筛选技术，从土壤中分离获得 18 株产木聚糖酶菌株，挑选其中 5 株进行摇瓶发酵。对产酶最高的菌株 S8-3 进行初步鉴定，同时分析该菌株产生的木聚糖酶的酶学性质。【结果】经初步鉴定该菌株为黑曲霉( Aspergillus niger) -1 ；该菌株发酵液的木聚糖酶活性高达628.43 U·mL ；木聚糖酶的最适作用温度为45℃，最适 pH为4.5，在 70℃保温 30 min 后酶活力仍为 60%以上，在 pH 3.0—7.0 内稳定性较好。【结论】预期该菌株产的木聚糖酶具有 用于饲料添加剂的潜力。 关键词：饲料；木聚糖酶；黑曲霉；酶学性质 Isolation of Microbial Strains Producing Acidic Xylanase and Characterization of the Enzyme GUO Chun-yan, DIAO Qi-yu, QIAO Yu, TU Yan, ZHANG Nai-feng, JIANG Cheng-gang (Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081 ) Abstract: 【Objective 】 This study was aiming at screening acidic xylanase-producing microbes from natural environment for potential use in feed industry. 【Method 】 Eighteen xylanase-producing strains were isolated from soil by enrichment culture techniques. Five strains were chosen to ferment in shaking flasks. Strain S8-3, which showed the highest xylanase production, was preliminarily identified by standard methods, and the property of the xylanase produced by the strain was studied. 【Result 】 Strain S8-3 was identified as Aspergillus niger . The maximal enzymatic activity of xylanase in culture solution was 628.43 U·mL-1. The xylanase activity preformed optimally at 45 ℃ and pH 4.5. The xylanase was stable at pH ranging from 3.0 to 7.0 and retained more than 60 % of its original activity after incuba