雌激素对卵巢切除术后小鼠的镇痛作用及对脊髓背-第三军医大学学报.docVIP

雌激素对福尔马林致痛后小鼠脊髓背角c-fos和P物质的影响 包晓航1，伊宏亮2，郭文琼2，范晓棠1 （400038 重庆，第三军医大学基础医学部：组织胚胎学教研室1，生理学教研室2） [摘要] 目的 观察雌激素对福尔马林致痛后小鼠痛觉评分及脊髓背角c-Fos和P物质表达的影响。方法 选用雌性C57/BL6小鼠15只，随机分成对照组（Control）、去卵巢手术组（OVX）、去卵巢手术雌激素组(OVX+E)结论 雌激素通过调节脊髓背角浅层感觉神经元的活动及痛觉纤维的传入参与对小鼠福尔马林痛的镇痛作用。 [关键词] 雌二醇疼痛测定原癌基因蛋白质c-fosP物质Effect of estrogen on the expression of c-fos and substance P in the dorsal horn of spinal cord in the mice following formalin stimulation Bao Xiaohang1, Yi Hongliang2, Guo Wenqiong2, Fan Xiaotang1 （1Department of Histology and Embryology, 2Department of Physiology, Third Military Medical University, Chongqing , 400038, China）[Abstract] Objective To observe the effect of estrogen on the pain score, c-fos and substance P expression in the dorsal horn of the spinal cord in the mice following formalin stimulation. Methods Fifteen C57/BL6 mice were randomized to three groups: Control group (intact mice without estrogen treatment), OVX+V group ( ovariectomized mice given vehicle) and OVX+E group(ovariectomized mice 17β-estradiol).Pain score was used to assay the role of estrogen in affecting pain threshold in the mice following formalin injected into the right hind paw, expression of c-fos and substance P in the dorsal horn of spinal cord (L3~L5) was tested with immunohistochemisty for evaluation neuron activity and pain afferent fibers. Result pain score was increased in ovariectomized mice following formalin stimulation, which can be inhibited by estrogen especially in the early stage of secondary phase. The number of c-Fos-like immunoreactivity neuron (FLIN) and substance P immunoreactivity in dorsal horn of the spinal cord was increased in ovariectomized mice , which can be reversed by estrogen. Conclusion Estrogen produced analgesic effect in OVX mice maybe mediate through modulating sensory neurons and afferent fibers in the superficial dorsal horn of the spinal cord. [Key words] Estradiol; ovariectomy; pain; pain measurement; spinal cord; proto-oncogene proteins c-fos; substance