大叶藻基因组铵根转运蛋白AMT的生物信息学特征_山东大学_威海_海洋学院_威海_.pdfVIP

网络出版时间：2017-09-11 15:24:02 网络出版地址：/kcms/detail/45.1369.Q1524.002.html 研究报告 Research Report 大叶藻基因组铵根转运蛋白 AMT 的生物信息学特征 * 吕新芳 于培 邓文浩 颜莹 段惠岚 李玉春 山东大学(威海)海洋学院, 威海, 264209 *通讯作者, li_yuchun@ 摘 要 本研究利用生物信息学方法，对大叶藻基因组中 8 个铵根转运蛋白(ZmAMTs)基因特征、氨基酸 组成成分、理化性质以及二级结构进行预测和分析，同时还分析了大叶藻与拟南芥、水稻、烟草及百脉根 的 AMT 基因家族之间的联系。大叶藻基因组中 8 个 AMT 基因分为 AMT1(5 个成员)和 AMT2(3 个成员) 两个子家族，所编码的蛋白质含有 9～11 个跨膜区，且均为疏水性蛋白质，二级结构均由 α螺旋、直链延 伸和无规卷曲三种形式构成。除此之外，大叶藻 AMT 家族蛋白具有相似的三维结构，多数成员定位于细 胞质、质膜和叶绿体膜上。本研究对大叶藻铵根转运蛋白进行了详细的生物信息学分析，可为今后深入研 究该家族基因的结构特征和功能提供参考。 关键词 大叶藻, 铵根转运蛋白, 生物信息学 Bioinformatical Characterization of Putative Ammonium Transporters in Zostera marina Genome Lv Xinfang Yu Pei Deng Wenhao Yan Ying Duan Huilan Li Yuchun Marine college, Shandong University at Weihai, Weihai, 264209 *Corresponding author, li_yuchun@ Abstract Through searching the protein database, 8 ammonium transport proteins (ZmAMTs) were identified from the genome of Zostera marina. Utilizing bioinformatic methods, we analyzed their gene length, amino acid composition, physical and chemical characteristics, as well as secondary structure prediction, and carried out phylogeny analyses of the 8 AMT proteins. Moreover, we assayed the evolutionary relationship of AMT gene families among Z. marina and Arabidopsis thaliana , Oryza sativa, Solanum lycopersicum, and Lotus japonicus . Homology alignment showed that ZmAMTs were classified into 2 sub-families, 5 members in AMT1 and 3 in AMT2. All of the AMT proteins contained multiple transmembrane domains, which AMT1 proteins had 9 transmembrane regions and AMT2 proteins had 11. All of the ZmAMT proteins were