大强度离心运动后热休克蛋白70在骨骼肌细胞中表达特征探究.docVIP

大强度离心运动后热休克蛋白70在骨骼肌细胞中表达特征探究 摘 要: 目的:观察离心运动后热休克蛋白70在骨骼肌细胞内的表达特征,探讨热休克蛋白70的保护作用机制。方法:成年雄性Wistar大鼠24只,随机分为对照组和运动组,运动组进行一次性大负荷离心运动,于运动后即刻和24 h取腓肠肌,冰冻切片,用腺苷三磷酸酶法和免疫荧光组织化学法观察热休克蛋白70的亚细胞分布特征。结果:安静状态热休克蛋白70分布在Ⅰ, IIa型肌纤维,IIb型肌纤维中没有分布。离心运动后热休克蛋白70从胞浆内移位到Ⅰ,IIa,IIb肌纤维的细胞膜和细胞核。结论:热休克蛋白70作为分子伴侣, 在肌纤维遭到应激时移位到细胞膜和核,可能对细胞膜起保护作用,也可能在膜损伤后,移 位于此帮助蛋白合成修复。 关键词:离心运动;热休克蛋白70;肌纤维类型 中图分类号:G804.2 文献标识码:A 文章编 号:1007-3612(2010)09-0046-03 Study on Heat Shock Protein 70 Expression Characteristic of Skel etal Muscle Cell after High?Intensity Eccentric Exercises GAO Qian?jin?1, MA Xin?dong?2 (1.Dept. of P.E. Shijiazhuang University, Shijiazhuang 050035,Hebei China;2. Tsinghua University, Beijing 100084, China) Abstract: Objective: The purpose of this study was to examine the characteristicexpression of hsp70 in skeletal muscle cell after the high?intensity eccentric exercise of rats once at a time. The mechanism protective effects of hsp70 in sk eletal muscle are observed. Methods: 24 adult male Wistar rats were divided intocontrol group (n=8) and exercise group. The exercise group did eccentric exerci ses at high intensity once at a time. After exercise, the rat gastrocnemius musc le was taken into cryo?section. And the hsp70 expression characteristic in diff e rent muscle fiber type was observed by the method of immunofluorence and matchro matic dye?ATPase. Result: Hsp70 has expression in Ⅰand IIa muscle fiber, and h a s no express in IIb muscle fiber. After eccentric exercise, Hsp70 transfer to Ⅰ ,IIa and IIb cell membrane and nucleus from cytoplasm. Conclusion: Hsp70 translo cation to cell membrane may be act as molecular chaperone to help proteins synth esis, and may be protect cell membrane against damage after cell suffer stress. Key words: eccentric exercise; Heat shock protein 70; muscle fiber typ e 反复的离心收缩可使骨骼肌肌纤维损伤,疼痛,用力困难。损伤机制有几种假说,包括 肌节和骨架蛋白破坏,细胞膜损伤,钙离子内环境破坏,炎症及氧化应激等。运动训练能 提高骨骼肌抗离心运动损伤的能力,被动伸展,等长收缩,和离心运动训练能使骨骼肌快速 产生适应,然而这种保护及适应的机制有待进一步阐明。骨骼肌含有被称为“分子伴侣”的 热休克蛋白