pIRES2-BDNF-VEGF165真核表达载体的构建与鉴定.pdfVIP

中国组织工程研究 笫 77眷 第 5D期 2013—12—10出版 ChineseJournalofTissueEngineenngResearch December10,2013 Vo1．17,No．50 WWW．CRTER．org doklO．3969／j．issn．2095-4344．2013．50．016 [http：／／www．crter．org] LiBN,LiWD,LinJT,FengHG ConstructionandIdentificationofplRES2一BDNF-VEGF185bicistroniceukaryoUcexpressionvector． ZhongguoZuzhiGongchengYanjiu．2013；17(50)：8719—8728． ConStruCtiOnandldentifiCationOf plRESz--BDNF--VEGF16sbicistroniceukaryotic expressionVeCtor水水☆ LiBing-nan，LiWei·dong，LinJun-tang，FengHui—gen DepartmentofLifeSconcesandTechnology,XinxiangMedicalUnivers-tyXinxiang453003．HenanProvinceChina Abstract BACKGROUND：Brain-denvedneurotrophicfactor(BDNF)andvascularendotheliaIgrowthfactor165 LiSing-nan☆ ，Ph．D ． (VEGFls5)areessentialgenesforcelldifferentiation．Virusmediatedmethodhasbeenusednumerouslyin Lecturer,DepartmentofLife SciencesandTechnology, researches，butthesecurityisthemostimportantproblem．Eukaryoticexpressingvectorisawaytosolvethis XinxiangMedicalUniversity, question． Xinxiang 453003。Henan Province，C：hina OBJECTIVE：ToconstructandidentifyplRES2-BDNF-VEGF165bicistroniceukaryoticexpressionvector． bingnanli120@yeahnet METHODS：BDNFgeneswereobtainedfr0m thegenomicDNA0fhumanperipheralbloodmononuclearcells byPCR．Then．theBDNFcDNAfragmentwasinsertedintolhemultiplecloningsitesofplRES2-EGFPto LiW ei—dong．Studyingfor