红外和拉曼光谱用于对丝蛋白构象的研究.PDFVIP

第 18 卷 第 11 期 化　学　进　展 Vol. 18 No. 11 2006 年 11 月 PROGRESS IN CHEMISTRY 　Nov. , 2006 红外和拉曼光谱用于对丝蛋白构象的研究 周　文　陈　新 　邵正中 (复旦大学高分子科学系 聚合物分子工程教育部重点实验室 　上海 200433) 摘 　要 　动物丝纤维和相关丝蛋白材料的性能与丝蛋白本身的二级结构即构象密切相关。红外光谱和 拉曼光谱是研究蛋白质构象的有力手段 ,因此在丝蛋白结构的研究中也有广泛的应用。本文综述了红外光 ( ) 谱和拉曼光谱在家蚕、野蚕 主要是柞蚕 和蜘蛛丝蛋白研究方面的应用 ,并对表征丝蛋白各种构象的红外和 拉曼特征峰进行了较为全面的归纳总结。 关键词 　桑蚕 　柞蚕 　蜘蛛 　构象转变 　酰胺 ( ) 中图分类号: O657. 3 ; Q51 　文献标识码 : A 　文章编号 : 1005281X 2006 Conformation Studies of Silk Proteins with Infrared and Raman Spectroscopy Zhou Wen 　Chen Xin 　Shao Zhengzhong ( Key Laboratory of Molecular Engineering of Polymers of Ministry of Education , Department of Macromolecular Science , Fudan University , Shanghai 200433 , China) Abstract 　The properties of silk fibers and relevant silk protein materials are highly related to the secondary structure , i. e. the conformation of silk protein itself. Both infrared and Raman spectroscopy are the powerful tools for the investigation of the protein conformations , so they are widely used in the study of silk proteins as well. In this review , the research of the structures on the domestic silkworm ( Bombyx mori ) , the wild silkworms (mainly tussah) as well as the spiders’silk proteins with the infrared and Raman spectroscopy are discussed , and the characteristic peaks of silk proteins related to the various conformations are summarized. Key words 　domestic silkworm ; tussah ; spider ; conformation transition ; amide