花楸叶活性成分提取工艺的优化 optimization of extraction process for active components from sorbus leaves.pdfVIP

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花楸叶活性成分提取工艺的优化 optimization of extraction process for active components from sorbus leaves.pdf

花楸叶活性成分提取工艺的优化 optimization of extraction process for active components from sorbus leaves

食品研究与开发 2014 年 2 月 分离提取 Food Research And Development 第 35 卷第 3 期 23 DOI :10.3969/j.issn.1005-6521.2014.03.007 花楸叶活性成分提取工艺的优化 柴军红,何婷婷,金志民,马怀良,肖杰,张鹏 (牡丹江师范学院,黑龙江 牡丹江157011) 摘 要 : 建立花楸叶中黄酮、多糖、萜类同步快速提取工艺,以期为花楸叶活性成分药用价值开发提供工艺基础。以 花楸叶为原料,以总黄酮、多糖、总萜提取率为技术指标,通过提取方法对比试验,采用正交设计与多指标综合分析法 > 乙醇浓度>pH(料液比)>酶添加量,优化最佳工 (权重)探讨提取工艺的影响因素。影响因素顺序依次是:超声波时间 艺参数:超声波时间 25 min , 乙醇浓度 50 % ,料液比 1 ∶ 25(g/mL ),pH=4 , 酶添加量 5 mg/g 。本工艺 3 种主要指标均比 传统提取法有很大提高,放大 20 倍实验表明工艺较为稳定,标准偏差均在 3 % 以内。 关键词: 花楸叶;黄酮;多糖;萜类;工艺优化 Optimization of Extraction Process for Active Components from Sorbus Leaves CHAI Jun-hong , HE Ting-ting ,JIN Zhi-min ,MA Huai-liang ,XIAO Jie ,ZHANG Ping (School of Science and Technology , Mu Dan Jiang Teachers College , Mudanjiang 157011 , Heilongjiang , China ) Abstract : To establish simultaneous rapid extraction process for flavonoids , polysaccharides , terpene from Sorbus leaves , it is expected to be promoted to active ingredients of Sorbus leaves to provide technology-based. Sorbus leaves was used as raw material , extraction rate of the total flavonoids , polysaccharides , total terpene are technical Index ; comparative study on extraction methods , using orthogonal design and comprehensive analysis ( ) : of multi -index weight to study on the effect of the extraction process. The factors affecting the order is ultrasonic time ethanol concentration pH (solid -liquid ratio ) enzyme added. The Optimal process parameters :ultrasonic tim

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