纤维素酶热稳定机制的计算分析 computational analysis of thermostability of cellulase.pdfVIP

现代食品科技 Modern Food Science and Technology 2009, Vol.25, No.6 纤维素酶热稳定机制的计算分析 曾沛斌，潘力 （华南理工大学生物科学与工程学院，广东 广州 510006） 摘要：对水解糖苷酶第45 家族的纤维素酶系进行计算分析,计算结果表明，与此家族酶系的热稳定性呈负相关的氨基酸对为：丙 氨酸-谷氨酸（AE ）、苯丙氨酸-赖氨酸（FK ）和丙氨酸-异亮氨酸（AI ）；呈正相关的氨基酸对：甲硫氨酸-脯氨酸（MP ）和甲硫氨酸- 甘氨酸（MG ），并引入氨基酸热稳定常量计算验证其相关性，发现呈负相关的氨基酸对均为显著相关，而呈正相关的氨基酸对显著 性不明显。这个结果可以为此家族纤维素酶的基因工程改造提供一定参考。 关键词：计算分析；氨基酸对；最适作用温度；纤维素酶；热稳定性 中图分类号：O658；文献标识码：A ；文章篇号:1673-9078(2009)06-0592-05 Computational Analysis of Thermostability of Cellulase ZENG Pei-bin, PAN Li （School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China ） Abstract: Two bioinformatic methods were combined to analyze the significant dipeptides responsible for optimum temperature of endoglucanases in Glycoside hydrolase in GHF 45. It was found that the negative amino acid pairs were Alanine-Glutamic acid, Phenylalanine-Lysine and Alanine-Isoleucine, which had significant physical-chemical, energetic and conformational properties that contribute to the thermostability of cellulase in this family, while the positive dipeptides were Methionine-Proline, and Methionine-Glycine, whose corresponding properties were not notable. The result was useful for cellulase engineering for high temperature activity. Key words: computational analysis, amino acid pair, optimum temperature, cellulase, thermostability 纤维素是地球上最为丰富的可再生性资源，如何 个氨基酸含量及氨基酸对进行多元回归分析，找出了 有效的开发和利用纤维素是当今生物领域的一个热点 与GHF45 中酶系的最适作用温度具有较高相关性的氨 [1] 。纤维素被彻底分解而又无污染的一条有效途径,就 基酸对，并引入氨基酸热稳定理化常量[9]，通过氨基酸 是利用纤维素酶的水解作用[2] 。寻求更高效能的纤维素 热稳定显著常量公式[10]进行计算，进一步分析认