HPLC法测定越橘益视胶囊中叶黄素的含量.pdfVIP

目 录 摘要 Ⅰ- Ⅱ 1 前言1 2 材料与仪器3 2.1 使用的主要试药及溶剂3 2.2 使用的主要仪器3 3 方法4 3.1 叶黄素的对照品溶液制备4 3.2 测定波长的选择4 3.3 供试品溶液的制备5 3.4 色谱条 5 3.5方法学考察5 4 讨论11 4.1 提取溶剂的选择11 4.2 色谱条件的选择12 4.3 方法学验证结果14 5 结论14 参考文献16 综 述17 致 谢22 HPLC法测定越橘益视胶囊中叶黄素的含量 摘要：目的 本文建立了简便易行、准确的HPLC- DAD法测定越橘益视胶囊中叶黄素的含量。方法 取样品0.3g，加水溶解后再加入含0.1%BHT的丙酮，在避光低温的条件下超声提取，定 容 过滤。色谱条件为资生堂MG Ⅱ色谱柱(4.6 mm×250 mm 5μm) ；流动相：甲醇- 甲基叔丁基醚（85:15）；流速：0.2 mL/min ，柱温：25℃ 检测波长445nm。结果 叶黄素在0.8488-42.44μg (r=0.9999)之间呈良好的线性关系，平均加样回收率为96.42% RSD为3.46%。结论 用高效液相色谱分离，二极管阵列检测器检测 测定叶黄素的方法精密度、稳定性等均 能满足保健食品中叶黄素分析要求，且灵敏度高，重现性好，操作简单快速，可作为越 橘益视胶囊中叶黄素含量测定方法。 关键词：越橘益视胶囊；叶黄素；高效液相色谱法；含量测定 I Determination of lutein in Yueju Yishi capsule by HPLC Abstract ：0bjective we systematically researched determination methods of lutein in Yueju Yishi capsule by HPLC-DAD, which is simple and accurate. Methods 0.3 gram sample with 5ml water to dissolve and then added about 40ml 0.1%BHT acetone was oscillated to extract the lutein in low temperature and filtrated by 0.45μm filter before sample injection for detection. The Shiseido MG II C18 column(4.6 mm×250 mm ，5μm) was used under the mobile phase of methanol-methyl tert-butyl enter (85:15,v/v)at a flow rate of 0.2mL/min. The column temperature was maintained at 25℃ and the detection wave length was 445nm. Results There was a good linear relationship at the ranges of 0.8488- 42.44μg for lutein(r=0.9999).The average recovery rate was 96.42% with relative standard deviation (RSD) of 3.46%. Conclusion The method with HPLC separation and DAD detection can meet the requirements for analysis of lutein in health care food with features of high precision ，high stability，simple and rapid ．It is suitable for determination of lutein in Yueju Yishi capsule. Keywords : Yueju Yishi capsule;Lutein;HPLC;Content determination