利用大孔吸附树脂纯化花生水解蛋白 Purification of peanut protein hydrolysates with macroporous adsorption resins.pdfVIP
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利用大孔吸附树脂纯化花生水解蛋白 Purification of peanut protein hydrolysates with macroporous adsorption resins
24 FATS 2010V01.35
CHINAOIl5AND No.12
油料蛋白
利用大孔吸附树脂纯化花生水解蛋白
王瑛瑶1,-,王璋2,栾霞1
(1.国家粮食局科学研究院,北京100037;2.江南大学食品学院,江苏无锡214122)
摘要:以水酶法制油工艺得到的花生水解蛋白为原料,从5种大孔吸附树脂中筛选出对花生水解蛋
白中可溶性肽有较好吸附效果的DA201一C作为纯化树脂对其进行纯化。静态吸附试验表明,
DA201一C对花生水解蛋白中可溶性肽有较好的吸附作用,能有效除去糖类杂质。DA201一C对可
溶性肽吸附平衡时间为3h,75%的乙醇解吸效果最好,解吸率为83.24%。动态试验结果显示,以
BV去离子水洗去糖类
pH为3的稀HAc溶液配制花生水解蛋白液能提高吸附率;解吸时,先用5
杂质,再收集
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