几种酶对鸭蛋蛋清水解效果的比较研究 Study on Reaction Condition for Enzymatic Hydrolysis of Duck Egg White by Some Kinds of Proteases.pdfVIP
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几种酶对鸭蛋蛋清水解效果的比较研究 Study on Reaction Condition for Enzymatic Hydrolysis of Duck Egg White by Some Kinds of Proteases
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几种酶对鸭蛋蛋清水解效果的比较研究
张建林,黄梅香,王海滨‘
(武汉工业学院食品科学与工程学院,湖北武汉430023)
摘要:为筛选利用鸭蛋蛋清酶解制备功能性多肽的条件,采用中性蛋白酶、碱性蛋白酶、木瓜蛋白酶和风味酶4种
酶对新鲜鸭蛋蛋清进行了水解。对每种酶分别进行单因素试验及正交试验,以水解度为指标确定每种酶的最佳水解
条件。结果表明,在各自的最佳水解条件下,木瓜蛋白酶的水解效果最好,其最佳水解条件为pH为9.5,水解温度为
50℃,底物浓度为25%,酶底比为8.0%,在此条件下水解度为29.01%。
关键词:鸭蛋蛋清;蛋白酶;水解条件;水解度
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