山苦茶多糖提取工艺及其部分成分分析 Extraction of Polysaccharides and Analysis of Ingredients in Mallotus furetianus Tea.pdfVIP
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山苦茶多糖提取工艺及其部分成分分析 Extraction of Polysaccharides and Analysis of Ingredients in Mallotus furetianus Tea
山苦茶多糖提取工艺及其部分成分分析
苏冰霞,葛会林,段云,叶海辉
(中国热带农业科学院分析测试中心,海南省热带果蔬产品质量安全重点实验室,海南海口 571101)
摘要:设计合理的超声波辅助提取山苦茶水溶性多糖的技术路线,Sevag试剂和蛋白酶结合脱蛋白,以水溶性多
糖得率为技术指标,正交试验得出最佳浸提工艺,并对样品的水分、灰分和15种元素含量进行分布分析。实验确定
水溶性山苦茶多糖的得率为9.976%。影响茶多糖浸出率的因素按影响大小依次为超声波功率超声时间超声温
度浸提时间液料比。蛋白质去除率为17.7%。山苦茶和茶渣等经烘箱干燥后均含有少量水分,可溶态灰分和不
Fe、Cu、Ca、Sc、Ni在茶渣中含量较高。
关键词:山苦茶多糖;提取工艺;元素分布
Extraction
of and of in Tea
PolysaccharidesAnalysisIngredientsMallotusfuretianus
SU Hui-lin,DUANYun,YEHai-hui
Bing-xia,GE
of Fruit
and and ofHainan of and
(KeyLaboratoryTropical Quality Province,Center
Vegetable Safety AnalysisTesting,
ofChinese 571
Academy AgriculturalSciences,Haikou
Tropical 101,China)
Abstract:Thisdescribesareasonableultrasonic·assisted forwater.soluble
paper extraction(UAE)techinque
Mallotus tea.Crude were thecombineduse
polysaccharides(WSPs)fromfuretianus polysaccharidesdeproteinizedby
of and conditionsforenhancedUAE of
extractionWSPswere250
Sevagreagentprotease.Theoptimumprocess W,40
30minforultrasonic ratioandhot
min,60℃,15:1(mL/g)and power,ultrasonicationtime,temperature,solid/liquid
waterextraction these
conditions,theextractionofWSPswas9.976%.Fiveextraction
time,respectively.U
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