山药苯丙氨酸解氨酶特性的研究 Study on the Characteristics of Phenylalanine Ammonia-lyase in Yam.pdfVIP

山药苯丙氨酸解氨酶特性的研究 江力-，袁怀波·，张世杰-，江汉湖： (1．合肥工业大学生物与食品工程学院，安徽合肥 230009； 2．南京农业大学食品科技学院，江苏南京 210095) 摘 要：本文研究了山药苯丙氨酸解氨酶(PAL)的动力学特性。结果表明，PAL最适底物浓度为1mmol，L，底物 浓度过高或过低都对酶活力不利。PAL在硼酸．氢氧化钠缓冲液中适宜的pH范围为7．6～9．2，最适pH有2个： 70℃则容易钝化。L．酪氨酸和L一半胱氨酸对PAL活性都有抑制作用，抑制程度随抑制剂浓度增加而增强。L．酪 氨酸的抑制作用较强，是PAL的竞争型抑制剂。L．胱氨酸对PAL的活性无影响。 关键词：山药；苯丙氨酸解氨酶；特性；抑制剂 of iIlY锄 onⅡle‰谢stics Study PhenylalaIlmeAmm衄ia-lyaSe JIANGLil，YUANHuai-b01，ZHANG Han-hu2 Shi-jiel，JIANG of Food of (1．Sch001 230009，Cllina； Biotecllnology卸dEngineering，HefeiUIliVersityTeclulology，Hefei ofFoodScienceand 210095，Cllina) 2．Couege Technology，N删ingA鲥cultIlmlUIliVers咄N砌iIlg Abstract：1nhekiIIedcch啪cterisdcsof smdied．111eresIIlts phenylalanine咖onia-lyase(PAL)iny锄waS sugges砌tllat of ofPALinH3802-NaoHbu雎rwasfrom me subs昀teconcen仃ationPALwas1I衄ol／L．ne op劬um optimaJpHmge 7．6to9．2觚dⅡle were collldIl’tbear出ali锄d mef0订Iler．The pH 8．O，8．8．PAL acid，especially optirrIaltempera嘛 op石mum rar姆eofPALw觞35℃t055℃锄dme叩tirnumtemperaturewas45℃，itsactivi哆waS to n也hadsometole舢ce had e雎ctson me lli曲temperatllre．L-哆rosine柚dL-cysteineinmbi血g y蛐PAL锄dinllibitory e插ectSmcreaSedwittlmeriseirItlleconcenmmonoft}IeiIllIibitors．Thein王Iibidon waSmore byL—tyrosiIle s们ng锄dappeared tobe w觞noef诧cton山e ofthePAL． competitive．L·cystine actiVit)