植物乳杆菌C8-1产类细菌素的初步研究 Preliminary characterization of bacteriocin-like substance formed by Lactobacillus plantarum C8-1.pdfVIP
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植物乳杆菌C8-1产类细菌素的初步研究 Preliminary characterization of bacteriocin-like substance formed by Lactobacillus plantarum C8-1
vrww.chinadaity.net 中国乳品工业
rpgy@chinajournal.net.cn dairy
植物乳杆菌C8—1产类细菌素的初步研究
张子健,刘玉恩,赵谦,张柏林
(河北农业大学食品科技学院,河北保定071000)
摘要:从发酵2周的泡菜中分离到66株乳杆茵.以其中4株乳杆菌作为出发菌株进行紫外诱变处理得到一株产类细茵素的突变株C8-
1。基于细胞形态、生理生化和16S
有较宽的抑茵谱.能够抑制大肠杆茵和金黄色葡萄球菌等多种食品腐败茵和致病菌;有较好的热稳定性,在4℃冷藏5d和一20℃冷冻5
oC和121℃分别加热15
d以)t-60,80,100
胃蛋白酶、木瓜蛋白酶耐受性较强。对胰蛋白酶较为敏感。这些数据表明,产类细菌素的植物乳杆菌C8—1在食品加工中具有进一步地
潜在应用价值。 .
关键词:类细菌素:植物乳杆茵:抑茵特性
中图分类号:Q93—33 文献标识码:A 文章编号:1001—2230(2010)01—0015—04
formed
characterizationofbacteriocin‘_-likesubstanceby
Preliminary
C8‘_。1
plantarum
ZHANG Bo—lin ·
Yu—en,ZHAOQian,ZHANG
Zi-jian,LIU
and of
ofFoodScience
(CoUege Technology,AgriculturalUniversityHebei,Baoding071001,cbJm)
fortwo isolateswereinducedUV
66isolatesofLactobacillus在omtraditionalChinese fermented
Abstract:Among vegetables weeks,four by
for theselactobacilli mutantC8—1was to abletOhave
bacteriocin.Fromfour strains,the
lightproducing provedproduceproteins
furtheridentifiedasLactobacillus toitscell and16S
C8—1WaS accordiIlg
activity.Strain plantarum
wide food and
rDNAdata.Bacteriocin—likesubstance strainC8—1hada antibacterialwhichinhibited
producedby spectrum spoiled
bacteria.Itshowed withinawide of at4。Corfrozenat一20oCfor5das
pathogenic goodstability rang
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