离子液体双水相萃取-hplc分析红酒中的痕量氯酚类物质.pdfVIP

260 2017, Vol.38, No.12 食品科学 ※安全检测 离子液体双水相萃取-HPLC分析红酒中的 痕量氯酚类物质 刘 曼，施 敏，曹学丽* （北京工商大学食品学院，北京食品营养与人类健康高精尖创新中心，北京 100048 ） 摘  要： [C MIM]BF (NH ) SO 6 建立亲水性离子液体 4 4 与无机盐 4 2 4 形成的离子液体双水相萃取富集红酒样品中 种痕量 氯酚类物质的方法。通过考察离子液体和盐的种类、pH值、离子液体和盐质量分数对氯酚萃取率和富集倍数的影 响，确定萃取氯酚的最优条件。在最佳萃取条件下，6 种氯酚的线性范围为20～200 μg/L ，相关系数（R2 ）达到 0.999，6 种氯酚的检出限在3.68～12.16 μg/L之间。对实际红酒样品进行测定，加标回收率为87.73%～103.44%，相 对标准偏差在0.33%～6.35%之间。该法操作简单、迅速、绿色且具有较高的萃取效率。 关键词：离子液体；双水相；氯酚；红酒；高效液相色谱 Determination of Trace Chlorophenols in Red Wine by Ionic Liquid Aqueous Two-Phase Extraction Coupled to HPLC * LIU Man, SHI Min, CAO Xueli (Beijing Advanced Innovation Center for Food Nutrition and Human Health, School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048, China) Abstract: An extraction method based on an aqueous two-phase system consisting of the hydrophilic ionic liquid [C4MIM] BF and the inorganic salt (NH ) SO for the simultaneous determination of six chlorophenols in red wine by high 4 4 2 4 performance liquid chromatography (HPLC) was established. The extraction efficiency and enrichment factor were found to be influenced by the types and concentrations of salts and ionic liquids, as well as pH value, and these parameters were investigated to determine the optimal conditions for extracting chlorophenols. Under the optimal conditions, the linear ranges of six chlorophenols were 20–200 μg/L with correlation coefficie