禽流感病毒H9N2亚型HA基因和传染性法氏囊病毒VP2基因真核表达型转移质粒的构建Construction of eukaryotic expression type transfer plasmid of avian influenza virus influenza subtype ha gene and infectious bursal virus VP2 gene.pdfVIP
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禽流感病毒H9N2亚型HA基因和传染性法氏囊病毒VP2基因真核表达型转移质粒的构建Construction of eukaryotic expression type transfer plasmid of avian influenza virus influenza subtype ha gene and infectious bursal virus VP2 gene
CONSTRUCTIoNOFEUKARYOTICTRANSFER
PLASMIDSEXPRESSING OF
AIVSUBTYPEH9N2oRIBDVVP2
By
LIUMiaomiao
Houchun
Supervisor:Prof.YAO
Prof.ZHANGcun
ADISSERTATIoN
Submittedto
NanjingAgriculturalUniversity
InPartialFulfillmentof
The forthe
Requirement
Master of Medicine
DegreeVeterinary
in
June,2012
Completed
CommencementinJune,2012
AIVH9N22.2.12.2.1.22.2.1.32.22.2.1
GSCVl988CVl988CVl98882.2.32.2.3.12.3.13.2.13.2.33.3.27783……………………………………………….2011
MDVVP2.CVl988
ABSTRACT
CONSTRUCTIONOFEUKARYOTICTRANSFER
PLASMIDS OFAIV
EXPRESSINGHEMAGGLUTININ
SUBTYPEH9N2ORIBDVVP2
ABSTRACT
The ofMarek’Sdisease withstrictcells
genome virus(MDV)ishuge combination,
thusthe ofconventionalmolecular is
application biologicaltechniquesinadequate.
the1 new DNA carrier
990s,a
Fortunately,in developedlarge-scalefragmentssystem,
artificial theMDVresearchconvenientand
bacterial quite
chromosome(BAC),makes
efficient.Withthis wasclonedintoBAC the
highly technology,MDVgenome vector,and
constructedMDVinfectionBACcloneCan and
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