新靶点CyclinE干扰RNA真核表达载体的构建.pdfVIP

L ab M ed Clin N ovem ber V ol N o C y clin E R N A CyclinE RNA U RT PCR mRNA CyclinE CyclinE RNA Lipofectamine U RT PCR CyclinEmRNA CyclinE RNA PCyclinE PCyclinE PCyclinE PCyclinE CyclinEmRNA CyclinE RNA Cy clinE RNA U CyclinE mRNA RNA E DOI 10 3969 issn2014 21 006 A j Construction of new target eukaryotic expression vectors of CyclinE siRNA QU Zhiz hen L I U Shan WA N G Y an y an GA O N aik ang H U Gej ile Dep artment of L abo ratory M e dic ine Dep artment of N euros urge ry A f f iliate d H osp ital of N eimengg u M e dical Univ e rs ity H uhehaote N e imeng g u China Abstract Objective T o construct the new targets eukaryotic ex pression vectors of human CyclinE interf er ence specific RNA to transf ect into glioma U cells the mRNA ex pression w as detected by RT PCR for obtaining the ukaryo tic ex pression vectors w ith the best interf erence effect to provide the valuable data for Cyclin E becoming the human tumor marker Methods Four new targets eukaryotic ex pression vectors of RNA interf erence specific CyclinE w ere constructed named as PCyclinE PCyclinE PCyclinE and PCyclinE T he double digestion method and the base sequence measuring w ere adopted to detect w hether the vectors w ere successf ully constructed T he four new ly constructed vectors w ere transfected into U cell line by Lipofectamine T he RT PCR results w as adopted to detect the ex pression quantity of CyclinE mRNA for selecting a vector w ith best interf erence Results new targets eukaryotic ex pression vectors of RNA interference specific for CyclinE w ere constructed CyclinEmRNA w as obviously suppressed the eukaryotic expression vector w ith best interference effec