ABO血型反定型梯形微板法的建立.doc

ABO血型反定型梯形微板法的建立 ? 284? ? 论着 中国输血杂志2007年8月第2O卷第4期ChinJBloodTransfusion,August,2007,Vo1.20,No.4 ABO血型反定型梯形微板法的建立 孙立平韩璐张庆武陈虹许婷婷(武汉血液中心,湖北武汉430030) 摘要:目的建立梯形微板ABO血型反定型检测方法.方法随机选用常规A,B,0,AB型血样各8份.运用 正交设计原理选择血浆稀释度,红细胞浓度,反应温度,静止时间4个参数的最佳条件;采用混合血样倍比稀释,确 定凝集与非凝集灰区值.根据已确定的最佳条件和灰区值,进行重复性试验,灵敏度试验,准确性试验,脂血干扰试 验,溶血干扰试验,并检测6份ABO亚型血样A,A,A.B,A.B,AB,BM和5份含不规则抗体血样抗～M,抗一N,抗一 P1,抗一Le,抗一HI,以评价对特殊血样的检出能力.结果重复性符合率100%.灵敏度可达到与试管法相同效价 的检出能力.准确性达99.99.47份不同脂血程度血样抗干扰试验一次判读正确率100.溶血干扰试验在Hb ≤20g/L时,对梯形微板法试验结果无明显影响,在严重溶血情况下(Hbgt;20g/L),对凝集端无影响,对非凝集端有 影响.对ll份特殊血样均有较好的检出能力.结论梯形微板法不仅能够很好地完成普通血样AB0反定型的检 测,还具有提高对弱凝集血样检测灵敏度的优势,进而提高试验的准确性. 关键词:AB0反定型;梯形微板法;U型微板法 中图分类号:R457.11R446.61文献标识码:A文章编号:1004—549X(2007)04—0284—03 Establishingatrapezoidmicro-platemethodtodetectABObloodgroupinserumSUNLiping,HANLu.zHANG Qingwu,eta1.WuhanBloodCenter,Wuhan,Hubei430030,China Abstract:ObjectiveEstablishingatrapezoidmicro～platemethodtodetectABObloodgroupinserum.Methods Select32bloodsamplescontainingA,B,OorABtypesrandomly.Useorthogonaldesignprincipletoselectthe optimumconditionsofthebloodplasmadilution,erythrocytesdensity,temperatureandstatictime.Themix—blood samplesareseriallydiluted.Theagglutinatingandnonagglutinatinggrayzonesarealsodefined.Basedontheopti- mumconditionsandcriterions,thereproducibditytest,sensitivenesstest,accuracytest,lipaemiainterferencetest andhaemolysisinterferencetestarecarriedOUt.6ABOsubgroup(AM,AX,A2B,A3B,AXB,BM)and5irregular antibody(anti--M.anti—N.anti—P1,anti—Leb,anti—HI)aredetectedtoevaluatetheabilityofthetrapezoidmi— cro—platemethodtodetectspecialsamples.ResultsThetrapezoidmicro—platemethodsrepetitivenesstestscoinci— denceis1OO.Itssensitivityisidenticaltothetesttubemethod.Theaccuracyis99.99.Inthe47variouskindsof lipaemiabloodsamplesanti—interferencetests.thecorrectinterpretationatonetimeis1OO.Inthehaemolysisin— terferencetests.whenhemoglobincontentarebelow20g/L.therearenoobviousinfluencesfound.Whenseverhae— molysisappears(bloodplasmahemoglobincontentareabove20g/L),thereisnoeffectontheagglutin