厌氧条件下微生物将磷还原为磷化氢-微生物学报.PDFVIP

微生物学通报 Jan. 20, 2015, 42(1): 34−41 Microbiology China © 2015 by Institute of Microbiology, CAS tongbao@ DOI: 10.13344/j.microbiol.china.140424 研究报告 厌氧条件下微生物将磷还原为磷化氢 王瑾丰1,2 牛晓君1,2* 马金玲1,2 陆美青1,2 (1. 510006) (2. 510006) 摘 要【目的】磷化氢为磷的气态形式，将污水中磷通过转化为磷化氢的方式去除，为污水 除磷提供新思路。【方法】采用厌氧持续培养的方式，以经过磷化氢处理和没有处理的水稻土分 别作为接种物，在氧化还原电位(ORP) −300 mV 、恒温35 °C ，避光持续培养160 d。【结果】培 养器1 出水总磷的去除率稳定达到25% ，最高去除率为26.78% ，气体磷化氢的产量达到130 ng/L 以上。培养器2 出水总磷去除达到23% ，气体磷化氢的产量达到126 ng/L。【结论】对水稻土 进行厌氧条件下连续培养，可以形成稳定的厌氧产磷化氢微生物体系，提高磷化氢的释放量。 关键词厌氧除磷，磷化氢，聚合酶链式反应-变性梯度凝胶电泳 Conversion of phosphorus to phosphine by microbial deoxidization under anaerobic conditions WANG Jin-Feng1,2 NIU Xiao-Jun 1,2* MA Jin-Ling 1,2 LU Mei-Qing1,2 (1. College of Environment and Energy, South China University of Technology, Guangzhou, Guangdong 510006, China) (2. The Key Laboratory of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, Guangzhou, Guangdong 510006, China) Abstract: [Objective] Conversion of phosphorus to phosphine, a gaseous form of phosphorus, has the potential to remove phosphorus from wastewater. [Methods] Incubator was kept anaerobically for 160 days, with paddy soil as inoculum that was treated either with phosphine without phosphine, under the condition ORP −300 mV, 35 °C and in dark. [Results] In incubator 1, total phosphorus removal in the effluent reached 25%, with the highest rate of 26.8%, and the gas phosphine output was above130 ng/L; and in incubator 2, total phosphorus removal in the effluent reached 23