多色细胞流式介绍.pdf

Selecting Reagents for Multicolor Flow Cytometry From HotLines Platinum Edition, Fall 2006 By Holden Maecker and Joe Trotter The availability of flow cytometers capable of detecting 6, 8, or Fluorochromes: Go for the bright more colors has spurred the discovery of new fluorochromes and Given the many differences in instrument configuration, it is impossible the development of antibody conjugates. However, the complexities to universally state the “best” fluorochromes to use in combinations of of choosing antibody combinations are such that simply using a 6, 8, or more colors. However, if one considers a particular cytometer random combination of fluorochromes for a particular set of antibody specificities is unlikely to provide optimal results. On the other hand, such as the BD™ LSR II instrument, it is possible to rank available dyes according to their brightness on that instrument (when configured with with a bit of forethought, one can usually avoid many rounds of trial a specified set of lasers and filters). But how exactly do we define and and error. The goal of this article is to provide some simple guidelines measure brightness? A good definition of brightness should probably to aid in the selection of reagent panels for multicolor flow cytometry, which should result in more successes than failures. start with resolution sensitivit