日本药典 No4 K-S.pdfVIP

JP XV O‹cial Monographs / Kainic Acid Hydrate 789789 1/15 mol/L phosphate buŠer solution, pH 5.6 to make ex- in a platinum crucible, dissolve in 5 mL of sodium carbonate actly 50 mL, and use this solution as the standard stock solu- TS, and evaporate on a water bath to dryness. Heat the cruci- tion. Keep the standard stock solution at 5 9C or below, and ble slowly at ˆrst, and then ignite until the sample is almost use within 3 days. Take exactly a suitable amount of the stan- incinerated. After cooling, add 12 mL of dilute nitric acid to dard stock solution before use, add 1/15 mol/L phosphate the residue, dissolve by warming, and ˆlter. Wash the residue buŠer solution, pH 5.6 to make solutions so that each mL with 15 mL of water, combine the washings and the ˆltrate, contains 80 mg (potency) and 20 mg (potency), and use these and add water to make 50 mL. Perform the test using this so- solutions as the high concentration standard solution and low lution as the test solution. concentration standard solution, respectively. Control solution: Add 5 mL of sodium carbonate TS to (iv) Sample solutions—Weigh accurately an amount of 0.30 mL of 0.01 mol L hydrochloric acid VS, and proceed as W Josamycin Propionate, equivalent to about 20 mg (potency), directed above (not more than 0.021z). dissolve in 10 mL of methanol, add 1/15 mol/L phosphate (3) Sulfate 1.14—Dissolve 0.5 g of Kainic Acid Hydrate buŠer solution, pH 5.6 to make exactly 50 mL. Take exactly in 40 mL of water by warming. Cool, add 1 mL of dilute a suitable amount of this solution, add