肝移植缺血再灌注损伤的比较蛋白质组学研究-中华肝胆外科杂志.PDFVIP

肝移植缺血再灌注损伤的比较 蛋白质组学研究 吴斌 陆敏强 [摘要]目的 研究肝移植手术中供体肝组织缺血一再灌注损伤的蛋白质变化规律。方法 2009年3月，对3例肝移植供肝于3个时间点切取肝组织标本进行对照研究：(1)新鲜的供肝组织; (2)经过缺血降温保存后修整完毕，但未经血管吻合的肝脏组织；(3)复流后的供肝组织。通过2DE- MALDI-TOF和质谱技术，对上述3个时间点的蛋白表达作出比较。结果 本研究共分离出1580个 蛋白点，其中与缺血再灌注损伤有关的蛋白19个。结合19个蛋白质的功能，对这些蛋白质的变化特 征进一步分析。结论 我们发现了与肝移植手术中供肝缺血性损伤和再灌注损伤相关的蛋白质，并 首次揭示了这些蛋白质在不同损伤阶段独立的变化规律。 肝移植； 缺血再灌注损伤； 蛋白质组学 Differentialprofeilproteomicofdonorliversubjecttoischemiaandreperfusioninjuryduringlivertrans plantationWU BinLUMin-qiangDepartmentofLiverTransplantation,the3rdAffiliatedHos pitalofSunYat-senUniversity,Guangzhou510630,P.R.China [Abstract] Objective Toidentifytheproteinspresentedvariationduringischemia/reperfusion injury(I/RI)inlivertransplantation(LT).Methods AtMar.2009,atotalofnineliversampletis sues,threesamplesobtainedfromeachdonorliveratthreedifferenttimepoints,werestudied.The timepointswereasfollows:(1)thetimepointimmediatelyafterdonorliverwasharvested(T1);(2) thetimepointjustbeforetheanastomosisofhepaticarteryandpotalvein(T2),duringthistimeperi odthedonorliverwaskeptinicecoldandtransported,thenback-tableprepared,duringtimefromT1 toT2,ischemiainjuryoccurred;(3)thetimeafterdonorreconnectedtotherecipient(T3),during whichthedonorliverwassubjectedtoreperfusioninjury.Thethreetimepointsrepresentnormalcon trol,ischemiainjury,andreperfusioninjuryrespectively.Byusingmethodof2DE-MALDI-TOFand mass-spectrum,thedifferentialexpressionoftheseproteinatT1,T2,andT3timepointwerecom pared.Results Fromthe1580proteinsthatwereisolated,19ischemia/reperfusioninjury(I/RI)-re latedproteinsthatvariedmarkedlyfromtimepointT1toT2andfromtimepointT2toT3wereiden tified.Theyweremetabolicenzyme,molecularchaperone,redoxase,cytoskeletonprotein,signal transductionprotein,andbindingprotein,respectively.Thecharacterofeachproteinwasfurtherana lizedbasedontheirfunctions.Conclusions Forthefirsttime,wedetectedtheproteinrelatedtoI/RI duringLT.Weobtainedindependentproteomicsinformationofischemiainjur