两株具促生作用的苜蓿内生菌的分离纯化与鉴定-微生物学报.PDF

微生物学通报 Feb. 20, 2015, 42(2): 280−288 Microbiology China © 2015 by Institute of Microbiology, CAS tongbao@ DOI: 10.13344/j.microbiol.china.140483 研究报告 两株具促生作用的苜蓿内生菌的分离纯化与鉴定 * * 康慧颖 王伟 刘佳莉 程园园 郭长虹 郭东林 (黑龙江省分子细胞遗传与遗传育种重点实验室 哈尔滨师范大学生命科学与技术科学学院 黑龙江 哈尔滨 150025) 摘 要：【目的】分离具有植物促生功能的苜蓿内生菌。【方法】从苜蓿的新鲜组织中分离纯化菌 株，进行 16S rRNA 基因序列分析，生理生化鉴定并检测内生菌对苜蓿的促生特性。【结果】获 得两株苜蓿内生菌，成团泛菌ASR16 和短小芽孢杆菌ALR33 。两株菌株均能产生植物生长激素 IAA 和嗜铁素，能溶解磷，对苜蓿的促生作用明显。【结论】获得两株具有植物促生特性的苜蓿 内生菌ASR16 和ALR33 ，可作为微生物菌肥的研究基础。 关键词：苜蓿，内生菌，促生 Isolation and identification of two plant-growth promoting endophytes from alfalfa * KANG Hui-Ying WANG Wei LIU Jia-Li CHENG Yuan-Yuan GUO Chang-Hong * GUO Dong-Lin (Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal University, Harbin , Heilongjiang 150025, China) Abstract: [Objective] To obtain endophytes which has the plant-growth promote function from alfalfa. [Methods] Two strains were isolated and purified from fresh tissues of alfalfa, and then analyzed by 16S rRNA gene sequence, identified by the physiological and biochemical methods. The plant-growth promote properties to alfalfa of two strains were detected. [Results] Two alfalfa endophytes, ASR16 (Pantoea vagans) and ALR33 (B